Department of Pathology, Kirikkale University, Faculty of Veterinary Medicine, 71451 Yahsihan, Kirikkale, Turkey.
Vet Parasitol. 2010 May 28;170(1-2):162-6. doi: 10.1016/j.vetpar.2010.01.045. Epub 2010 Feb 7.
Echinococcus granulosus, the causative agent of cystic echinococcosis, not only often causes unilocular cysts in intermediate hosts, but also in rare cases induces formation of multivesicular cysts which have similar morphology to alveolar cysts. The aim of the present study was to characterise multivesicular and unilocular hydatid cysts in cattle using morphologic and molecular diagnostic tools. Multivesicular cysts were detected in 4 out of 1255 slaughtered cows. Four unilocular cysts were also included in the study to compare with multivesicular cyst morphology. For histopathological evaluation, tissues were fixed in 10% neutral formalin. Following a routine histological tissue-processing procedure, samples were embedded in paraffin blocks and serial sections were cut at a thickness of 4-5 microm. For polymerase chain reaction (PCR), cyst walls and/or protoscolices recovered from six materials were preserved in 70% alcohol. Histopathologically, severity of calcification, fibrous capsule formation and giant cell layer were similar for multivesicular and unilocular cysts. However, the severity of subcapsular inflammation, inflammatory cell infiltration into adjacent organ parenchyma and eosinophil leucocyte infiltration into the cyst lumen was higher in multivesicular cysts. PCR analyses revealed that all unilocular hydatid cysts as well as two out of four multivesicular cysts were G1 genotype of E. granulosus. Molecular diagnosis of the other two multivesicular structures remained inconclusive as DNAs obtained from paraffin-embedded cyst walls were fragmented to small parts, as short as 100 bp, which were not suitable for PCR analyses. In conclusion, molecular analysis concomitant to histopathological examinations is useful in differential diagnosis of multivesicular echinococcosis.
细粒棘球蚴（Echinococcus granulosus）是导致包虫病（cystic echinococcosis）的病原体，不仅经常在中间宿主中引起单房性囊肿，而且在罕见情况下还会诱导形成具有肺泡样囊肿相似形态的多房性囊肿。本研究旨在使用形态学和分子诊断工具来描述牛的多房性和单房性包虫囊肿。在 1255 头屠宰牛中，发现了 4 个多房性囊肿。为了与多房性囊肿形态进行比较，还纳入了 4 个单房性囊肿。为了进行组织病理学评估，组织固定在 10%中性福尔马林中。经过常规组织处理程序，将样本嵌入石蜡块中，并以 4-5 微米的厚度切割连续切片。为了进行聚合酶链反应（PCR），从 6 种材料中回收的囊壁和/或原头节保存在 70%酒精中。组织病理学上，多房性和单房性囊肿的钙化、纤维囊形成和巨细胞层严重程度相似。然而，多房性囊肿的囊下炎症、炎症细胞浸润邻近器官实质和嗜酸性白细胞浸润囊腔的严重程度更高。PCR 分析显示，所有单房性包虫囊肿以及 4 个多房性囊肿中的 2 个均为细粒棘球蚴的 G1 基因型。对另外两个多房性结构的分子诊断仍不确定，因为从石蜡包埋的囊壁中获得的 DNA 被碎片化到非常小的片段，最短可达 100bp，不适合 PCR 分析。总之，形态学检查结合分子分析在多房性包虫病的鉴别诊断中是有用的。
