Department of Pharmacology, Toxicology and Pharmacy University of Veterinary Medicine Hannover, Germany.
J Biomater Appl. 2011 Mar;25(7):685-97. doi: 10.1177/0885328209360424. Epub 2010 Mar 5.
Degradable magnesium alloys are new materials for implants used in orthopedic and trauma surgery. The aim of this study was to investigate the influence of degradable magnesium alloys on the function of dendritic cells (DC) as these cells represent the major antigen presenting cells of the body. MgP (pure magnesium), MgCa 0.6 (0.6% calcium), MgCa 0.8 (0.8% calcium), MgCa 1.0 (1% calcium), and MgCa 1.2 (1.2% calcium) alloys were degraded in cell culture medium. In parallel, murine bone marrow-derived DC were incubated with increasing concentrations (0.1-10 mmol/L) of magnesium chloride and calcium chloride, respectively. Incubation of DC with degradation media over 6 days had no influence on cell viability and only marginal influence on DC migration. Also, the production of TNFα and expression of CD86 was not enhanced by incubation with degraded magnesium alloys. The mixed leukocyte reaction revealed that there was also no increase of the T-cell proliferation in comparison to untreated controls. However, there was a trend toward macrophage development at the expense of DC expansion and an enhanced DC migration was induced by incubation with higher magnesium concentrations. Particularly the latter should be verified in in vivo experiments.
可降解镁合金是用于骨科和创伤外科植入物的新型材料。本研究旨在研究可降解镁合金对树突状细胞（DC）功能的影响，因为这些细胞是机体主要的抗原呈递细胞。MgP（纯镁）、MgCa0.6（0.6%钙）、MgCa0.8（0.8%钙）、MgCa1.0（1%钙）和 MgCa1.2（1.2%钙）合金在细胞培养基中降解。同时，用不同浓度（0.1-10mmol/L）的氯化镁和氯化钙孵育鼠骨髓来源的 DC。孵育 6 天的 DC 与降解培养基接触对细胞活力没有影响，对 DC 迁移只有轻微影响。而且，用降解镁合金孵育也不会增强 TNFα 的产生和 CD86 的表达。混合淋巴细胞反应显示，与未处理的对照组相比，T 细胞增殖也没有增加。然而，与 DC 扩张相比，培养物中存在向巨噬细胞发育的趋势，并且用较高浓度的镁孵育可诱导 DC 迁移增加。特别是后者应在体内实验中进行验证。
