细胞对可生物降解镁合金在人生长板软骨细胞和成骨细胞中的反应。
Cellular reactions to biodegradable magnesium alloys on human growth plate chondrocytes and osteoblasts.
机构信息
Department of Orthopaedic Surgery, Medical University of Graz, Auenbruggerplatz 5, 8036, Graz, Austria,
出版信息
Int Orthop. 2014 Apr;38(4):881-9. doi: 10.1007/s00264-013-2163-3. Epub 2013 Nov 21.
PURPOSE
In recent decades operative fracture treatment using elastic stable intramedullary nails (ESINs) has mainly taken precedence over conservative alternatives in children. The development of biodegradable materials that could be used for ESINs would be a further step towards treatment improvement. Due to its mechanical and elastic properties, magnesium seems to be an ideal material for biodegradable implant application. The aim of this study was therefore to investigate the cellular reaction to biodegradable magnesium implants in vitro.
METHODS
Primary human growth plate chondrocytes and MG63 osteoblasts were used for this study. Viability and metabolic activity in response to the eluate of a rapidly and a slower degrading magnesium alloy were investigated. Furthermore, changes in gene expression were assessed and live cell imaging was performed.
RESULTS
A superior performance of the slower degrading WZ21 alloy's eluate was detected regarding cell viability and metabolic activity, cell proliferation and morphology. However, the ZX50 alloy's eluate induced a favourable up-regulation of osteogenic markers in MG63 osteoblasts.
CONCLUSIONS
This study showed that magnesium alloys for use in biodegradable implant application are well tolerated in both osteoblasts and growth plate chondrocytes respectively.
目的
近几十年来，弹性稳定髓内钉（ESIN）的手术骨折治疗主要优先于保守治疗。可用于 ESIN 的生物可降解材料的发展将是治疗改进的又一步。由于其机械和弹性特性，镁似乎是生物可降解植入物应用的理想材料。因此，本研究旨在体外研究生物可降解镁植入物的细胞反应。
方法
本研究使用原代人生长板软骨细胞和 MG63 成骨细胞。研究了对快速和缓慢降解镁合金浸提液的细胞活力和代谢活性的影响。此外，还评估了基因表达的变化并进行了活细胞成像。
结果
与细胞活力和代谢活性、细胞增殖和形态相比，快速降解 WZ21 合金浸提液的性能更优。然而，ZX50 合金的浸提液诱导了 MG63 成骨细胞中成骨标志物的上调。
结论
本研究表明，用于生物可降解植入物应用的镁合金在成骨细胞和生长板软骨细胞中分别具有良好的耐受性。
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