Universidad de Antioquia, Medellín, Colombia.
Am J Trop Med Hyg. 2010 Mar;82(3):402-11. doi: 10.4269/ajtmh.2010.09-0464.
Thick film, the standard diagnostic procedure for malaria, is not always ordered promptly. A failsafe diagnostic strategy using an XE-2100 analyzer is proposed, and for this strategy, malaria diagnostic models for the XE-2100 were developed and tested for accuracy. Two hundred eighty-one samples were distributed into Plasmodium vivax, P. falciparum, and acute febrile syndrome groups for model construction. Model validation was performed using 60% of malaria cases and a composite control group of samples from AFS and healthy participants from endemic and non-endemic regions. For P. vivax, two observer-dependent models (accuracy = 95.3-96.9%), one non-observer-dependent model using built-in variables (accuracy = 94.7%), and one non-observer-dependent model using new and built-in variables (accuracy = 96.8%) were developed. For P. falciparum, two non-observer-dependent models (accuracies = 85% and 89%) were developed. These models could be used by health personnel or be integrated as a malaria alarm for the XE-2100 to prompt early malaria microscopic diagnosis.
厚涂片是疟疾的标准诊断程序，但并不总是能及时进行。本文提出了一种使用 XE-2100 分析仪的可靠诊断策略，并针对该策略开发和测试了用于 XE-2100 的疟疾诊断模型，以评估其准确性。将 281 份样本分为间日疟原虫、恶性疟原虫和急性发热综合征组，用于模型构建。使用 60%的疟疾病例和来自发热综合征和来自流行和非流行地区的健康参与者的复合对照组样本进行模型验证。对于间日疟原虫，开发了两种依赖观察者的模型（准确性=95.3-96.9%）、一种使用内置变量的非依赖观察者模型（准确性=94.7%）和一种使用新的和内置变量的非依赖观察者模型（准确性=96.8%）。对于恶性疟原虫，开发了两种非依赖观察者的模型（准确性分别为 85%和 89%）。这些模型可由卫生人员使用，或作为 XE-2100 的疟疾警报集成，以提示尽早进行疟疾显微镜诊断。
