School of Chemical and Biological Engineering, Seoul National University, 599 Gwanangno, Gwanakgu, Seoul 151-742, Korea.
Small. 2010 Apr 9;6(7):872-9. doi: 10.1002/smll.200902074.
Poly(3,4-ethylenedioxythiophene) (PEDT) is recognized as one of the most promising conducting polymers for future applications in the fields of electronics, optics, energy storage/conversion, and biomedical science. The toxicity of PEDT could be considered to affect the potential for its widespread application. Herein, the cytotoxicity and proinflammatory response of PEDT nanomaterials of three different shapes toward human lung fibroblast (IMR90) and mouse alveolar macrophage (J774A.1) cells are investigated. The shape-dependent toxicity of the PEDT nanomaterials is evaluated by examining cell morphological change, cytotoxicity, apoptosis/necrosis, oxidative stress, and immune response. The cytotoxicity and apoptosis of the nanomaterials increase with their decreasing aspect ratio in both cell lines. The formation of reactive oxygen species in cells treated with PEDT nanomaterials is dependent on the shape and concentration of the nanomaterial. Proinflammatory cytokines, such as interleukin-1, interleukin-6, and tumor necrosis factor alpha from macrophages, are induced by PEDT nanomaterial-treated cells.
聚(3,4-乙撑二氧噻吩) (PEDT) 被认为是最有前途的导电聚合物之一，有望在电子学、光学、储能/转换和生物医学科学领域得到广泛应用。PEDT 的毒性可能会影响其广泛应用的潜力。在此，研究了三种不同形状的 PEDT 纳米材料对人肺成纤维细胞 (IMR90) 和小鼠肺泡巨噬细胞 (J774A.1) 的细胞毒性和促炎反应。通过观察细胞形态变化、细胞毒性、细胞凋亡/坏死、氧化应激和免疫反应，评估了 PEDT 纳米材料的形状依赖性毒性。在两种细胞系中，纳米材料的细胞毒性和细胞凋亡随其纵横比的降低而增加。细胞内活性氧的形成取决于纳米材料的形状和浓度。巨噬细胞中白细胞介素 1、白细胞介素 6 和肿瘤坏死因子α等促炎细胞因子被 PEDT 纳米材料处理的细胞诱导产生。
