Simon T L, Sierra E R, Ferdinando B, Moore R
Department of Pathology, University of New Mexico School of Medicine, Albuquerque.
Transfusion. 1991 May;31(4):335-9. doi: 10.1046/j.1537-2995.1991.31491213299.x.
A new apheresis device using microprocessor control for the collection of a high-purity single-donor platelet concentrate was evaluated, as was the storage of platelets for up to 5 days in a citrate-plasticized polyvinylchloride blood bag. The study was conducted in three phases: collection of platelets for in vitro studies and determination of donor safety; autologous transfusion of platelets in healthy volunteers; and transfusion of platelets in patients requiring platelet transfusion therapy. Donors had mild hypocalcemia and minimal changes in blood counts except for a platelet count reduction from 288 +/- 50 x 10(3) (288 +/- 50 x 10(9)/L) to 217 +/- 43 x 10(3) per microL (217 +/- 43 x 10(9)/L). A mean of 3.36 +/- 1.24 x 10(11) platelets was collected in the mean volume of 214 mL with red cell and white cell contamination in the range of 10(7). Morphology and aggregation were as described previously in stored platelets. Platelet survival data in eight subjects showed a mean recovery of 61 +/- 11 percent and mean survival of 5.03 +/- 1.07 days by a weighted-mean model. Patients transfused with platelets had mean increments of 23,000 immediately and of 8000 at 24 hours; corrected count increments were 6000 at 1 hour and 4000 at 24 hours. The platelets were successful in providing hemostasis to these patients. Clinically useful 5-day-stored platelets are obtained by using this apheresis technology with a functionally closed system and a citrate-plasticized blood bag.
对一种使用微处理器控制来采集高纯度单供体血小板浓缩物的新型血液成分分离装置进行了评估，同时也对血小板在柠檬酸盐增塑聚氯乙烯血袋中储存长达5天的情况进行了评估。该研究分三个阶段进行：采集血小板用于体外研究并确定供体安全性；在健康志愿者中进行血小板自体输血；以及在需要血小板输血治疗的患者中进行血小板输血。供体出现轻度低钙血症，除血小板计数从每微升288±50×10³（288±50×10⁹/L）降至217±43×10³外，血细胞计数变化极小。平均采集到3.36±1.24×10¹¹个血小板，平均体积为214 mL，红细胞和白细胞污染在10⁷范围内。形态和聚集情况如先前所述的储存血小板。八名受试者的血小板存活数据显示，通过加权平均模型，平均回收率为61±11%，平均存活时间为5.03±1.07天。接受血小板输血的患者立即平均增加23000，24小时后增加8000；校正计数增加在1小时时为6000，24小时时为4000。这些血小板成功地为这些患者提供了止血作用。通过使用这种具有功能封闭系统和柠檬酸盐增塑血袋的血液成分分离技术，可以获得临床上有用的储存5天的血小板。
