Rock G, Tittley P, McCombie N
Ottawa Centre Canadian Red Cross, Blood Transfusion Service, Ontario, Canada.
Transfusion. 1989 May;29(4):288-91. doi: 10.1046/j.1537-2995.1989.29489242791.x.
Platelets were collected from normal donors via a blood cell separator (Fenwal CS-3000). Platelets were stored initially in two separate 1000-ml bags (average count per bag, 2.3 +/- 0.5 x 10(11)) in 100 ml of autologous plasma for 5 days. Little change in platelet count was noted after 5 days of storage; however, the white cell count fell from 5.1 +/- 1.7 x 10(9) at Time 0 to 3.4 +/- 2.3 x 10(9) per l at Day five. The initial lactate values were 32 +/- 11 mg per dl and rose to 165 +/- 28 mg per/dl by 5 days. Platelet aggregation was impaired both by the collection procedure and during storage: whereas the response to ADP of the donors' platelets before the procedure was 100 percent, samples taken from the product immediately after collection had only a 45 percent response, which fell to 12 percent by Day 5. Aggregation using epinephrine was similarly affected, with a 75 percent response after collection and 0 percent response by Day 5. The plasma beta-thromboglobulin (beta-TG) level was high, both after collection (5.0 micrograms/ml at Time 0) and after storage (11.0 micrograms/ml), indicating a considerable effect of collection on platelet alpha granule release. In vivo recovery of these platelets was very good at 67 +/- 6 percent, with an average survival of 7.3 +/- 1.4 days (multiple hit; n = 4).(ABSTRACT TRUNCATED AT 250 WORDS)
通过血细胞分离机（芬瓦CS - 3000）从正常供体采集血小板。血小板最初储存在两个单独的1000毫升袋中（每袋平均计数为2.3±0.5×10¹¹），置于100毫升自体血浆中保存5天。储存5天后血小板计数变化不大；然而，白细胞计数从第0天的5.1±1.7×10⁹降至第5天的3.4±2.3×10⁹/升。初始乳酸值为32±11毫克/分升，到5天时升至165±28毫克/分升。血小板聚集在采集过程中和储存期间均受到损害：采集前供体血小板对ADP的反应为100%，采集后立即从产品中取出的样本反应仅为45%，到第5天降至12%。使用肾上腺素的聚集也受到类似影响，采集后反应为75%，到第5天反应为0%。血浆β - 血小板球蛋白（β - TG）水平在采集后（第0天为5.0微克/毫升）和储存后（11.0微克/毫升）均较高，表明采集对血小板α颗粒释放有相当大的影响。这些血小板的体内回收率非常好，为67±6%，平均生存期为7.3±1.4天（多次打击；n = 4）。（摘要截于250字）
