Nanospectra Biosciences, Incorporated, Houston, Texas 77054, USA.
J Biomed Opt. 2010 Jan-Feb;15(1):018001. doi: 10.1117/1.3290817.
The photothermal ablation of solid tumors using exogenous, near-infrared (NIR)-absorbing nanoparticles has been previously investigated using various preclinical models and is currently being evaluated in the clinic. Here, we evaluate the circulation kinetics, preliminary toxicity, and efficacy of photothermal ablation of solid tumors using gold nanorods systemically delivered and passively accumulated in a murine subcutaneous colon cancer model. Tumored animals were infused with nanorods followed by the percutaneous illumination of the tumor with an 808-nm laser. Control groups consisted of laser-only, nanorod-only, and untreated tumored animals. The survival of the treated and control groups were monitored for 60 days post-treatment. The survival of the photothermally treated group was statistically longer than the control groups, with approximately 44% tumor free through the evaluation period. Histopathology of the major organs of animals infused with nanorods did not indicate any significant toxicity at 60 days post-treatment. Particle biodistribution was evaluated by elemental analysis of the major organs of untumored mice at 1, 7, and 30 days after infusion with nanorods. Elemental analysis indicates nanorod clearance from the blood and retention by the reticuloendothelial system. This study indicates that gold nanorods are promising agents for photothermal ablation of solid tumors.
利用外源近红外（NIR）吸收纳米粒子对实体瘤进行光热消融已在各种临床前模型中进行了研究，目前正在临床中进行评估。在此，我们评估了金纳米棒系统给药和被动积累在小鼠皮下结肠癌模型中对实体瘤的光热消融的循环动力学、初步毒性和疗效。肿瘤动物接受纳米棒输注后，用 808nm 激光经皮照射肿瘤。对照组包括激光组、纳米棒组和未经处理的肿瘤动物组。治疗组和对照组的动物在治疗后 60 天内进行生存监测。光热治疗组的生存率明显长于对照组，在评估期内约有 44%的肿瘤未复发。在治疗后 60 天，对输注纳米棒的动物的主要器官进行组织病理学检查，未显示出任何明显的毒性。在输注纳米棒后 1、7 和 30 天，对未肿瘤小鼠的主要器官进行元素分析，评估了粒子的生物分布。元素分析表明纳米棒从血液中清除并被网状内皮系统保留。这项研究表明，金纳米棒是实体瘤光热消融的有前途的药物。
