Molecular Cardiology and Angiogenesis Laboratory, Department of Surgery, University of Connecticut Health Center, Farmington, CT, USA.
Mol Cell Biochem. 2010 Jul;340(1-2):73-80. doi: 10.1007/s11010-010-0402-0. Epub 2010 Mar 7.
Calcium is an essential mineral to support bone health and serves as a major therapeutic intervention to prevent and delay the incidence of osteoporosis. Many individuals do not obtain the optimum amount of calcium from diets and depend on bioavailable calcium supplements. The present study was conducted to examine the effect of a novel plant-based calcium supplement, derived from marine algae, and contains high levels of calcium, magnesium, and other bone supporting minerals [commercially known as AlgaeCal (AC)], on proliferation, mineralization, and oxidative stress in cultured human osteoblast cells, and compared with inorganic calcium carbonate and calcium citrate salts. Cultured human fetal osteoblast cells (hFOB 1.19) were treated with AC (0.5 mg/ml, fixed by MTT assay), calcium carbonate, or calcium citrate. These cells were harvested after 4 days of treatment for ALP activity, PCNA expression, and DNA synthesis, and 2 days for Ca(2+) deposition in the presence and absence of vitamin D3 (5 nM). The ability of AC to reduce H(2)O(2) (0.3 mM)-induced oxidative stress was assessed after 24 h of treatment. ALP activity was significantly increased with AC treatment when compared to control, calcium carbonate, or calcium citrate (4.0-, 2.0-, and 2.5-fold, respectively). PCNA expression (immunocytochemical analysis), DNA synthesis (4.0-, 3.0-, and 4.0-fold, respectively), and Ca(2+) deposition (2.0-, 1.0-, and 4.0-fold, respectively) were significantly increased in AC-treated cells when compared with control, calcium carbonate, or calcium citrate treatment. These markers were further enhanced following additional supplementation of vitamin D3 in the AC-treated group cells. AC treatment significantly reduced the H(2)O(2)-induced oxidative stress when compared to calcium carbonate or calcium citrate (1.5- and 1.4-fold, respectively). These findings suggest that AC may serve as a superior calcium supplement as compared to other calcium salts tested in the present study. Hence, AC may be developed as a novel anti-osteoporotic supplement in the near future.
钙是支持骨骼健康的必需矿物质，也是预防和延缓骨质疏松症发生的主要治疗干预措施。许多人无法从饮食中获得最佳量的钙，因此依赖于可利用的钙补充剂。本研究旨在检查一种新型的植物源钙补充剂（来源于海藻）的效果，该补充剂含有高水平的钙、镁和其他支持骨骼的矿物质[商业上称为藻钙（AC）]，对培养的人成骨细胞的增殖、矿化和氧化应激的影响，并与无机碳酸钙和柠檬酸钙盐进行比较。用 AC（0.5mg/ml，通过 MTT 测定固定）、碳酸钙或柠檬酸钙处理培养的人胎成骨细胞（hFOB 1.19）。处理 4 天后收获细胞，用于碱性磷酸酶（ALP）活性、增殖细胞核抗原（PCNA）表达和 DNA 合成，2 天后在有或无维生素 D3（5 nM）的情况下测定 Ca2+沉积。处理 24 小时后，评估 AC 降低 H2O2（0.3 mM）诱导的氧化应激的能力。与对照组、碳酸钙或柠檬酸钙相比，AC 处理显著增加了 ALP 活性（分别增加 4.0、2.0 和 2.5 倍）。与对照组、碳酸钙或柠檬酸钙相比，AC 处理的细胞中 PCNA 表达（免疫细胞化学分析）、DNA 合成（分别增加 4.0、3.0 和 4.0 倍）和 Ca2+沉积（分别增加 2.0、1.0 和 4.0 倍）显著增加。在 AC 处理组细胞中添加维生素 D3 后，这些标志物进一步增强。与碳酸钙或柠檬酸钙相比，AC 处理显著降低了 H2O2 诱导的氧化应激（分别减少 1.5 和 1.4 倍）。这些发现表明，与本研究中测试的其他钙盐相比，AC 可能是一种更好的钙补充剂。因此，AC 可能在不久的将来开发为一种新型的抗骨质疏松补充剂。
