Liu Guo, Guo Baoyan, Sun Shengwei, Luo Minna, Liu Fei, Miao Jianyin, Tang Jian, Huang Yahui, Cao Yong, Song Mingyue
Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou, China.
College of Horticulture, South China Agricultural University, Guangzhou, China.
Front Nutr. 2021 Aug 30;8:743791. doi: 10.3389/fnut.2021.743791. eCollection 2021.
Casein phosphopeptides have been studied widely for their ability to chelate calcium. However, systematic studies on the effects of casein phosphopeptides (CPP) on calcium absorption and are scarce. The purities of two commercially available products, CPP1 and CPP2, are 18.37 and 25.12%, respectively. Here, the calcium binding capacity of CPP2 was 142.56 ± 7.39 mg/g, which was higher than that of CPP1 (107.15 ± 6.27 mg/g). The calcium transport results in a Caco-2 monolayer model indicated that, relative to controls, CPP1 and CPP2 increased calcium transport by 21.78 and 53.68%, respectively. Subsequent animal experiments showed that the CPP2-Ca-H group (1% Ca, 0.4% CPP2) had significant increases in the femur index, serum Ca and serum osteocalcin levels, and femoral Ca content. The CPP2-Ca-H animal also had decreased serum alkaline phosphatase levels, parathyroid hormone content, and urinary pyridinoline content. Overall, our results demonstrated that CPP2 had stronger effects on promoting calcium uptake than CPP1.
酪蛋白磷酸肽因其螯合钙的能力而受到广泛研究。然而，关于酪蛋白磷酸肽（CPP）对钙吸收影响的系统研究却很匮乏。两种市售产品CPP1和CPP2的纯度分别为18.37%和25.12%。在此，CPP2的钙结合能力为142.56±7.39mg/g，高于CPP1（107.15±6.27mg/g）。在Caco-2单层模型中的钙转运结果表明，相对于对照组，CPP1和CPP2分别使钙转运增加了21.78%和53.68%。随后的动物实验表明，CPP2-Ca-H组（1%钙，0.4%CPP2）的股骨指数、血清钙和血清骨钙素水平以及股骨钙含量显著增加。CPP2-Ca-H组动物的血清碱性磷酸酶水平、甲状旁腺激素含量和尿吡啶啉含量也有所降低。总体而言，我们的结果表明，CPP2在促进钙吸收方面比CPP1具有更强的作用。
