Módis László, Szalai Eszter, Németh Gábor, Berta András
Department of Ophthalmology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary.
Eur J Ophthalmol. 2010 Sep-Oct;20(5):831-8. doi: 10.1177/112067211002000504.
The study was conducted to assess the central corneal thickness (CCT) of the healthy cornea with a recently developed noncontact specular microscope (EM-3000; Tomey) and compare the results with those measured with a contact specular microscope and an ultrasound pachymeter. Agreement between measurements taken by 2 investigators was also studied.
The right eyes of 41 healthy individuals who had negative history of contact lens wear, ophthalmic disease, or ocular surgery were examined. The CCT was determined sequentially with a noncontact specular microscope, a contact specular microscope (EM-1000; Tomey), and an ultrasound pachymeter (AL-2000; Tomey). Each evaluation with the specular microscopes was performed by 2 independent operators.
A significant difference was detected in pachymetry measurements among the 3 instruments (p=0.01; analysis of variance). The mean CCT values were lower measured with the ultrasound pachymeter (537+/-30 microm) than the contact endothelial microscope (543+/-37 microm, p=0.17, Student t-test) and the noncontact microscope (549+/-33 microm, p<0.0001) (operator 1). There was no statistically significant difference in CCT measurements between the 2 endothelial microscopes (p=0.19). We found significant correlations (p<0.0001) in thickness measurements between each pair of instruments (r=0.91, noncontact microscopy and ultrasound pachymetry; r=0.74, noncontact and contact microscopy; r=0.72, contact microscopy and ultrasound pachymetry; Spearman rank correlation).
The strong correlations among the 3 pachymetry devices suggest that the tested instruments provide reliable measurements; however, they cannot be used interchangeably.
本研究旨在使用一种新开发的非接触式角膜内皮显微镜（EM - 3000；多美）评估健康角膜的中央角膜厚度（CCT），并将结果与使用接触式角膜内皮显微镜和超声角膜测厚仪测量的结果进行比较。同时也研究了两名检查者测量结果之间的一致性。
对41名无隐形眼镜佩戴史、眼科疾病或眼部手术史的健康个体的右眼进行检查。依次使用非接触式角膜内皮显微镜、接触式角膜内皮显微镜（EM - 1000；多美）和超声角膜测厚仪（AL - 2000；多美）测定CCT。角膜内皮显微镜的每次评估均由两名独立操作人员进行。
三种仪器测量的角膜厚度存在显著差异（p = 0.01；方差分析）。超声角膜测厚仪测量的平均CCT值（537±30微米）低于接触式角膜内皮显微镜（543±37微米，p = 0.17，学生t检验）和非接触式显微镜（操作人员1测量为549±33微米，p < 0.0001）。两种角膜内皮显微镜测量的CCT之间无统计学显著差异（p = 0.19）。我们发现每对仪器测量的厚度之间存在显著相关性（p < 0.0001）（r = 0.91，非接触显微镜与超声角膜测厚仪；r = 0.74，非接触与接触显微镜；r = 0.72，接触显微镜与超声角膜测厚仪；Spearman等级相关）。
三种角膜测厚设备之间的强相关性表明所测试的仪器能够提供可靠的测量结果；然而，它们不能互换使用。
