利用带有 DNA 适体的微流控芯片分析仪快速检测利钠肽：利钠肽检测的应用。
Rapid detection of natriuretic peptides by a microfluidic LabChip analyzer with DNA aptamers: Application of natriuretic peptide detection.
出版信息
Biomicrofluidics. 2009 Jul 30;3(3):34101. doi: 10.1063/1.3194283.
Abstract
Rapid detection of brain natriuretic peptide (BNP) concentration can be used for the diagnosis of acute heart failure and for the evaluation of the effectiveness of a clinical therapy. We used the systematic evolution of ligands by exponential enrichment method to develop DNA aptamers for BNP whose sequences were determined by cloning method and consensus sequence analysis. A total of eight conserved sequences was identified. By combining the fluorescent-labeled aptamers with fast protein lab-on-chip analysis, we could achieve quantification of BNP concentrations with high speed, sensitivity, and specificity.
摘要
快速检测脑利钠肽（BNP）浓度可用于急性心力衰竭的诊断，并可用于评估临床治疗的效果。我们使用指数富集的配体系统进化（SELEX）方法，通过克隆方法和一致序列分析确定了针对 BNP 的 DNA 适体序列。共鉴定出 8 个保守序列。通过将荧光标记的适体与快速蛋白质芯片分析相结合，我们可以实现快速、灵敏和特异性地定量 BNP 浓度。
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