Department of Materials Science and Technology, Tokyo University of Science, 2641 Yamazaki, 278-8510 Chiba, Japan.
J Phys Chem B. 2010 Apr 1;114(12):4358-65. doi: 10.1021/jp911607h.
Gadolinium hydroxide, Gd(OH)(3), nanostructures were examined for their possible use in imaging and tracking of cells and tissues by investigating their cellular interactions and cytotoxic behaviors. For this purpose, Gd(OH)(3) nanorods (length, several hundred nanometers; diameter, approximately 40 nm) and spherical nanoparticles (average diameter, <10 nm) were synthesized by solvothermal decomposition of gadolinium containing molecular precursors. After comprehensive characterization of material properties, human colon adenocarcinoma (Caco2) and human lung epithelial (A549) cells were incubated with Gd(OH)(3) nanostructures in concentrations up to 900 microg/mL to perform cytotoxicity assays (BrdU (5-bromo-2'-deoxyuridine), WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzol-disulfonate)) and living/dead staining. As observed in all in vitro assays, the investigated Gd(OH)(3) nanostructures do not induce any significant cytotoxic effect, making them promising candidates for a new class of contrast agents, which may overcome the limitations of organic stains such as photobleaching and single usage.
研究了氢氧化钆（Gd(OH)(3)）纳米结构在细胞和组织成像和跟踪方面的潜在应用，考察了其细胞相互作用和细胞毒性行为。为此，通过溶剂热分解含钆的分子前体合成了 Gd(OH)(3)纳米棒（长度几百纳米；直径约 40nm）和球形纳米颗粒（平均直径<10nm）。在对材料性质进行了全面表征后，将人结肠腺癌细胞（Caco2）和人肺上皮细胞（A549）与高达 900μg/mL 的 Gd(OH)(3)纳米结构孵育，以进行细胞毒性测定（BrdU（5-溴-2'-脱氧尿苷），WST-1（4-[3-(4-碘苯基)-2-(4-硝基苯基)-2H-5-四唑基]-1,3-苯并二磺酸钠））和活/死染色。在所有体外实验中观察到，所研究的 Gd(OH)(3)纳米结构不会引起任何明显的细胞毒性作用，这使得它们成为一类新的对比剂的有前途的候选物，这些对比剂可能克服有机染料如光漂白和单次使用的局限性。
