Siberian Institute of Medical and Environmental Research, Angarsk, Russia.
Bull Exp Biol Med. 2023 Sep;175(5):695-699. doi: 10.1007/s10517-023-05928-6. Epub 2023 Oct 20.
We studied the cytotoxic effect of gadolinium nanocomposite on cultured mouse fibroblasts 3T3-SV40 and histological changes in the liver tissue of albino rats after its administration. For in vitro experiment, gadolinium nanocomposite on the natural matrix of arabinogalactan (nGd-AG) was dissolved in DMEM nutrient medium to concentrations of 0.005, 0.02, 0.5, 2, and 5 mM. In in vivo experiment, a nGd-AG solution was orally administered to rats through a tube in a dose of 500 μg Gd/kg in 1 ml of 0.9% NaCl for 10 days. The pattern and degree of influence of the gadolinium nanocomposite on the studied cell culture depended on the concentration and duration of exposure. IC of nGd-AG determined after cell incubation for 24, 48, and 72 h were 616 μg/kg (3.9 mM), 302 μg/kg (1.9 mM), and 222 μg/kg (1.4 mM), respectively. Histological changes in the liver of white rats induced by exposure to nanocomposite attested to the development of a compensatory reaction of the organ.
我们研究了钆纳米复合材料对培养的小鼠成纤维细胞 3T3-SV40 的细胞毒性作用，以及给药后白化大鼠肝组织的组织学变化。在体外实验中，将天然阿拉伯半乳聚糖基质上的钆纳米复合材料（nGd-AG）溶解在 DMEM 营养培养基中，浓度分别为 0.005、0.02、0.5、2 和 5mM。在体内实验中，通过管将 nGd-AG 溶液以 500μg Gd/kg 的剂量口服给予大鼠，在 1ml 0.9%NaCl 中，连续 10 天。钆纳米复合材料对研究细胞培养的影响模式和程度取决于暴露的浓度和持续时间。细胞孵育 24、48 和 72 小时后测定的 nGd-AG 的 IC 分别为 616μg/kg（3.9mM）、302μg/kg（1.9mM）和 222μg/kg（1.4mM）。暴露于纳米复合材料后，白色大鼠肝组织的组织学变化证明了器官的代偿反应的发展。
