Center for Technologies against Cancer, Tokyo University of Science, 2669 Yamazaki, 278-0022 Chiba, Japan.
Acta Biomater. 2013 Jan;9(1):4734-43. doi: 10.1016/j.actbio.2012.08.045. Epub 2012 Sep 7.
Bioimaging is an important diagnostic tool in the investigation and visualization of biological phenomena in cells and in medicine. In this context, up-converting Gd(2)O(3):Er(3+),Yb(3+) nanostructures (nanoparticles, nanorods) have been synthesized by precipitation methods and hydrothermal synthesis. Independent of size and morphology, Gd(2)O(3):Er(3+),Yb(3+) powders show up-conversion (550 nm, 670 nm) and near-infrared emission (1.5 μm) upon 980 nm excitation, which makes these structures interesting for application as biomarkers. With regard to their potential application in bioimaging, cytotoxicity is an important aspect and is strongly affected by the physico-chemical properties of the investigated nanostructures. Therefore, the cytotoxic effect of bare and poly(ethylene glycol)-b-poly(acrylic acid) block co-polymer-modified nanostructures on non-phagocytic and phagocytic cells (B-cell hybridoma cells and macrophages) was investigated. The observed cytotoxic behavior in the case of macrophages incubated with bare nanostructures was assigned to the poor chemical durability of gadolinium oxide, but could be overcome by surface modification.
生物成像是细胞和医学中研究和可视化生物现象的重要诊断工具。在此背景下，通过沉淀法和水热合成合成了上转换 Gd(2)O(3):Er(3+),Yb(3+) 纳米结构（纳米粒子、纳米棒）。无论尺寸和形态如何，Gd(2)O(3):Er(3+),Yb(3+) 粉末在 980nm 激发下都会显示上转换（550nm、670nm）和近红外发射（1.5μm），这使得这些结构在作为生物标志物方面很有趣。关于它们在生物成像中的潜在应用，细胞毒性是一个重要方面，并且强烈受研究纳米结构的物理化学性质的影响。因此，研究了裸纳米结构和聚（乙二醇）-b-聚（丙烯酸）嵌段共聚物修饰纳米结构对非吞噬细胞和吞噬细胞（B 细胞杂交瘤细胞和巨噬细胞）的细胞毒性。与巨噬细胞孵育时观察到的裸纳米结构的细胞毒性行为归因于氧化钆的化学稳定性差，但可以通过表面改性来克服。
