Ohtsubo M, Yoshida T, Seino H, Nishitani H, Clark K L, Sprague G F, Frasch M, Nishimoto T
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Fukuoka, Japan.
EMBO J. 1991 May;10(5):1265-73. doi: 10.1002/j.1460-2075.1991.tb08068.x.
The RCC1 gene has been isolated from several vertebrates, including human, hamster and Xenopus. Genes similar to RCC1, namely BJ1 and SRM1/PRP20, have been isolated from the insect Drosophila and from the budding yeast Saccharomyces cerevisiae. A mutation of the RCC1 gene in the hamster BHK21 cell line, tsBN2, confers pleiotropic phenotypes, including G1 arrest and premature induction of mitosis in cells synchronized at the G1/S boundary. Similarly, mutations of the SRM1/PRP20 gene are pleiotropic; the srm1 mutant shows G1 arrest and suppression of the mating defect of mutants lacking pheromone receptors, and the prp20 mutant shows an alteration in mRNA metabolism. Here we show that both BJ1 and SRM1/PRP20 complement the temperature sensitive phenotype of the tsBN2 cells. Like RCC1 proteins of vertebrates, the protein products of the Drosophila and yeast RCC1 homologues were located in the nuclei of the mammalian cells. These results suggest that the BJ1 and SRM1/PRP20 genes are functionally equivalent to the vertebrate RCC1 genes, and that the RCC1 gene plays an important role in the regulation of gene expression in the eukaryotic cell cycle.
RCC1基因已从包括人类、仓鼠和非洲爪蟾在内的多种脊椎动物中分离出来。与RCC1相似的基因，即BJ1和SRM1/PRP20，已从昆虫果蝇和芽殖酵母酿酒酵母中分离出来。仓鼠BHK21细胞系tsBN2中的RCC1基因突变会导致多效性表型，包括在G1/S边界同步化的细胞中出现G1期停滞和有丝分裂的过早诱导。同样，SRM1/PRP20基因的突变也是多效性的；srm1突变体表现出G1期停滞并抑制缺乏信息素受体的突变体的交配缺陷，而prp20突变体则表现出mRNA代谢的改变。在这里，我们表明BJ1和SRM1/PRP20都能互补tsBN2细胞的温度敏感表型。与脊椎动物的RCC1蛋白一样，果蝇和酵母RCC1同源物的蛋白质产物位于哺乳动物细胞的细胞核中。这些结果表明，BJ1和SRM1/PRP20基因在功能上等同于脊椎动物的RCC1基因，并且RCC1基因在真核细胞周期的基因表达调控中起重要作用。
