Keerl Silke, Gehmert Sebastian, Gehmert Sanga, Song Yao-Hua, Alt Eckhard
Department of Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA.
Ann Plast Surg. 2010 Apr;64(4):487-90. doi: 10.1097/SAP.0b013e31819f3a3d.
Recent evidence indicates that mesenchymal stem cells contribute to tumor angiogenesis through yet undefined mechanisms. In the present study, we investigated the angiogenic properties of human adipose tissue-derived stem cells and the mechanisms involved. Freshly isolated human adipose tissue-derived stem cells were seeded onto wells coated with Matrigel and cultured in endothelial growth medium. Capillary-like tube formation was observed after 18 hours culture. Tube formation was significantly reduced in the presence of antibodies against platelet-derived growth factor receptor beta or basic fibroblast growth factor. Collectively, these data suggest that freshly isolated adipose tissue-derived stem cells have the capacity to differentiate into capillary structures and platelet-derived growth factor and bFGF plays a critical role in this process.
近期证据表明，间充质干细胞通过尚未明确的机制促进肿瘤血管生成。在本研究中，我们调查了人脂肪组织来源干细胞的血管生成特性及其相关机制。将新鲜分离的人脂肪组织来源干细胞接种到包被有基质胶的孔中，并在内皮细胞生长培养基中培养。培养18小时后观察到毛细血管样管腔形成。在存在抗血小板衍生生长因子受体β或碱性成纤维细胞生长因子的抗体时，管腔形成显著减少。总体而言，这些数据表明，新鲜分离的脂肪组织来源干细胞具有分化为毛细血管结构的能力，血小板衍生生长因子和碱性成纤维细胞生长因子在此过程中起关键作用。
