Koenen Paola, Spanholtz Timo A, Maegele Marc, Stürmer Ewa, Brockamp Thomas, Neugebauer Edmund, Thamm Oliver C
Clinic for Plastic Surgery, Reconstructive- and Handsurgery, Burn Care Center, University of Witten/Herdecke, Cologne-Merheim Medical Center, Cologne, Germany; Department of Trauma and Orthopaedic Surgery, University of Witten/Herdecke, Cologne-Merheim Medical Center, Cologne, Germany; Institute for Research in Operative Medicine, University of Witten/Herdecke, Cologne, Germany.
Int Wound J. 2015 Feb;12(1):10-6. doi: 10.1111/iwj.12039. Epub 2013 Mar 13.
Wound healing is a complex biological process that requires a well-orchestrated interaction of mediators as well as resident and infiltrating cells. In this context, mesenchymal stem cells play a crucial role as they are attracted to the wound site and influence tissue regeneration by various mechanisms. In chronic wounds, these processes are disturbed. In a comparative approach, adipose-derived stem cells (ASC) were treated with acute and chronic wound fluids (AWF and CWF, respectively). Proliferation and migration were investigated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and transwell migration assay. Gene expression changes were analysed using quantitative real time-polymerase chain reaction. AWF had a significantly stronger chemotactic impact on ASC than CWF (77·5% versus 59·8% migrated cells). While proliferation was stimulated by AWF up to 136·3%, CWF had a negative effect on proliferation over time (80·3%). Expression of b-FGF, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 was strongly induced by CWF compared with a mild induction by AWF. These results give an insight into impaired ASC function in chronic wounds. The detected effect of CWF on proliferation and migration of ASC might be one reason for an insufficient healing process in chronic wounds.
伤口愈合是一个复杂的生物学过程，需要多种介质以及驻留细胞和浸润细胞之间精心协调的相互作用。在这种情况下，间充质干细胞起着关键作用，因为它们被吸引到伤口部位，并通过各种机制影响组织再生。在慢性伤口中，这些过程会受到干扰。采用比较方法，分别用急性和慢性伤口渗出液（AWF和CWF）处理脂肪来源的干细胞（ASC）。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验和Transwell迁移试验研究细胞增殖和迁移情况。使用定量实时聚合酶链反应分析基因表达变化。与CWF相比，AWF对ASC的趋化作用明显更强（迁移细胞分别为77.5%和59.8%）。虽然AWF可将细胞增殖刺激至136.3%，但随着时间的推移，CWF对增殖有负面影响（80.3%）。与AWF的轻度诱导相比，CWF强烈诱导碱性成纤维细胞生长因子（b-FGF）、血管内皮生长因子（VEGF）和基质金属蛋白酶-9的表达。这些结果深入了解了慢性伤口中ASC功能受损的情况。检测到的CWF对ASC增殖和迁移的影响可能是慢性伤口愈合过程不足的一个原因。
