Castells M T, Ballesta J, Madrid J F, Aviles M, Martinez-Menarguez J A
Department of Cell Biology, Medical School, University of Murcia, Spain.
Histochemistry. 1991;95(4):419-26. doi: 10.1007/BF00266971.
The glycoconjugates of the respiratory system of rats from 15 days of gestation through the adult period have been characterized by means of both conventional and lectin histochemistry. The main changes occurred at 20-21 days of gestation immediately before birth. An increase of acidic groups in the glycoproteins of the lung and airway epithelium was observed by conventional mucin histochemistry. The combined use of neuraminidase digestion and lectin histochemistry demonstrated an increase of sialic acid residues at the terminal position of the glucidic moieties of the glycoproteins. The sialic acid residues were linked alpha (2-3, 6) to D-galactose (beta 1-3)-N-acetylgalactosamine, thus masking the PNA-reactivity detected on the luminal surface of Clara cells and pneumonocytes before birth. In the adult period, alpha-L-fucose residues, detected by UEA-I, were localized in the glycoproteins contained in goblet cells and periciliary layer of the rat airway epithelium. The modifications observed in the lung of developing rats are similar to those previously described in human fetal and neonatal lungs. This suggests that the rat represents a useful model to study the glycoprotein synthesis during lung development.
通过传统组织化学和凝集素组织化学方法，对从妊娠15天到成年期大鼠呼吸系统的糖缀合物进行了表征。主要变化发生在出生前即妊娠20 - 21天。通过传统的粘蛋白组织化学观察到肺和气道上皮糖蛋白中酸性基团增加。神经氨酸酶消化和凝集素组织化学联合使用表明，糖蛋白糖基部分末端位置的唾液酸残基增加。唾液酸残基以α(2 - 3, 6)连接到D - 半乳糖(β1 - 3)-N - 乙酰半乳糖胺，从而掩盖了出生前在克拉拉细胞和肺细胞腔表面检测到的PNA反应性。在成年期，通过UEA - I检测到的α - L - 岩藻糖残基定位于大鼠气道上皮杯状细胞和纤毛周层所含的糖蛋白中。在发育中大鼠肺中观察到的变化与先前在人类胎儿和新生儿肺中描述的变化相似。这表明大鼠是研究肺发育过程中糖蛋白合成的有用模型。
