Orgambide G, Montrozier H, Servin P, Roussel J, Trigalet-Demery D, Trigalet A
Centre de Recherche de Biochimie et de Génétique Cellulaires, Toulouse, France.
J Biol Chem. 1991 May 5;266(13):8312-21.
The exopolysaccharide of Pseudomonas solanacearum, which is believed to play an important role in bacterial virulence, was considered by most authors as a homogeneous entity essentially composed of N-acetylgalactosamine. The present work demonstrates the high degree of heterogeneity of this exopolysaccharidic material, which consists of a high molecular weight acidic polysaccharide and a mainly noncarbohydrate structure as major subfractions. Rhamnose-rich polyoside and glucan fractions are also present as minor components. We report the complete structure of the acidic heteropolymer involving, in addition to N-acetylgalactosamine, equimolar ratios of two rare amino sugars, 2-N-acetyl-2-deoxy-L-galacturonic acid and 2-N-acetyl-4-N-(3-hydroxybutanoyl)-2,4,6-trideoxy-D-glucose. The structure of this acidic exopolysaccharide provides the first precise basis for the analysis of the correlation exopolysaccharide structure with pathogenicity in P. solanacearum.
青枯假单胞菌的胞外多糖被认为在细菌致病性中起重要作用，大多数作者认为它是一种基本上由N - 乙酰半乳糖胺组成的均质物质。目前的研究表明这种胞外多糖物质具有高度的异质性，它由一种高分子量酸性多糖和一种主要为非碳水化合物结构作为主要亚组分组成。富含鼠李糖的多聚糖苷和葡聚糖组分也作为次要成分存在。我们报道了这种酸性杂聚物的完整结构，除了N - 乙酰半乳糖胺外，还涉及两种稀有氨基糖的等摩尔比，即2 - N - 乙酰 - 2 - 脱氧 - L - 半乳糖醛酸和2 - N - 乙酰 - 4 - N -（3 - 羟基丁酰基）- 2,4,6 - 三脱氧 - D - 葡萄糖。这种酸性胞外多糖的结构为分析青枯假单胞菌中胞外多糖结构与致病性之间的相关性提供了首个精确依据。
