Laboratorio di Analisi Ospedale Filippo Del Ponte, Azienda Ospedaliera Ospedale di Circolo e Fondazione Macchi, Via Filippo Del Ponte 19, 21100 Varese, Italy.
Clin Chim Acta. 2010 Jun 3;411(11-12):882-5. doi: 10.1016/j.cca.2010.03.008. Epub 2010 Mar 11.
Harmonization of results allows a more effective utilization of laboratory tests; we verified the feasibility of harmonizing serum alkaline phosphatase results by two methods.
Patient sera (n=106) and candidate calibration materials (n=8) were analyzed by two methods, employing either diethanolamine (DEA) or 2-amino-2-methyl-1-propanol (AMP) as phosphate-accepting buffers. Results for patient sera by the DEA method were recalculated, with either a commutable or a non-commutable calibration material, both with values assigned by the AMP method.
After calibration with the commutable material, the median intermethod difference (DEA-AMP) and ratio (DEA/AMP) dropped from 195 U/l to 0 U/l and from 2.47 to 1.00, respectively. When a non-commutable material was used the former became 124 U/l and the latter 1.94. After recalibration with the commutable material, linear regression and correlation analysis of DEA vs AMP values for the set of 106 patient sera gave: intercept=0.8 U/l; slope=0.997; and nonparametric correlation coefficient r=0.9995.
Harmonization of alkaline phosphatase results by AMP and DEA methods is feasible when commutable calibration materials are used in the trueness transfer process.
结果的协调化可使实验室检测得到更有效的利用；我们通过两种方法验证了使血清碱性磷酸酶结果协调化的可行性。
用两种方法分析了 106 份患者血清和 8 份候选校准材料，使用的磷酸接受缓冲液分别为二乙醇胺（DEA）或 2-氨基-2-甲基-1-丙醇（AMP）。用 DEA 法对患者血清的结果进行重新计算，采用可互换或不可互换的校准材料，均用 AMP 法赋值。
用可互换材料校准后，两种方法间的中位数差值（DEA-AMP）和比值（DEA/AMP）从 195 U/l 降至 0 U/l，从 2.47 降至 1.00。使用不可互换材料时，前者变为 124 U/l，后者变为 1.94。用可互换材料重新校准后，对 106 份患者血清的 DEA 与 AMP 值进行线性回归和相关分析，得到：截距=0.8 U/l；斜率=0.997；非参数相关系数 r=0.9995。
当在真实转移过程中使用可互换校准材料时，用 AMP 和 DEA 方法使碱性磷酸酶结果协调化是可行的。
