Mondal B, Rasool T J, Ram Hira, Mallanna S
Indian Veterinary Research Institute, Mukteswar, Dist. Nainital, Uttaranchal-263138, India.
Biologicals. 2010 May;38(3):401-6. doi: 10.1016/j.biologicals.2010.01.016. Epub 2010 Mar 12.
Duck virus enteritis (DVE) also known as duck plague, is a viral infection of ducks caused by duck enteritis virus (DEV). The control of the disease is mainly done by vaccination with a chicken embryo-adapted live virus that is known to be poorly immunogenic and affords partial protection. Further, the risk of harboring other infectious agents in the embryo particularly the deadly and zoonotic avian influenza virus is also high. In this paper, we report propagation of a chicken embryo-adapted vaccine strain of duck enteritis virus in duck embryo fibroblast (DEF) cell line. Thirty serial passages were done in DEF cell that made the vaccine virus further attenuated which was tested in ducks. The growth behaviors of the virus in DEF cells were studied and at 30th passage level the virus titre was found to be 10(6.8) TCID(50)/ml. Ducks were immunized with this virus and challenged after 21 days with high dose of virulent DEV. All the immunized ducks withstood challenge with no clinical symptoms in any of the ducks while all the control ducks died. DEF cell which is free from other infectious agents appears to be a good system for cultivation of duck enteritis virus vaccine strain.
鸭病毒性肠炎（DVE），也称为鸭瘟，是一种由鸭肠炎病毒（DEV）引起的鸭病毒性感染。该病的防控主要通过接种鸡胚适应的活病毒疫苗来进行，已知这种疫苗免疫原性较差且只能提供部分保护。此外，鸡胚中携带其他传染源尤其是致命的人畜共患禽流感病毒的风险也很高。在本文中，我们报道了鸭肠炎病毒的鸡胚适应疫苗株在鸭胚成纤维细胞（DEF）系中的增殖情况。在DEF细胞中进行了30次连续传代，使疫苗病毒进一步减毒，并在鸭身上进行了试验。研究了该病毒在DEF细胞中的生长特性，在第30代时病毒滴度为10(6.8) TCID(50)/ml。用这种病毒对鸭进行免疫，21天后用高剂量的强毒DEV进行攻毒。所有免疫鸭均耐受攻毒，无任何临床症状，而所有对照鸭均死亡。不含其他传染源的DEF细胞似乎是培养鸭肠炎病毒疫苗株的良好系统。
