Department of Preclinic and Clinic Pharmacology, University of Firenze, Firenze, Italy.
J Immunol. 2010 Apr 15;184(8):4258-68. doi: 10.4049/jimmunol.0902972. Epub 2010 Mar 12.
In this study, we present evidence of differential Th17 responses in human monocyte-derived dendritic cells exposed to the pathogenic Candida albicans or the nonpathogenic Saccharomyces cerevisiae. We use different forms of the microorganisms, cells, hyphae, and spores, as a toolbox to dissect the role of surface mannan in the fungal immune response. In contrast to the S. cerevisiae yeast cell-induced Th1 response, dendritic cells stimulated with spores or C. albicans hyphae induce cellular responses shifted toward Th17 differentiation. The differential recognition of specific mannan structures is the master regulator of the discrimination between harmful and harmless fungi. The switch between spores and yeast is crucial for the commensalism of S. cerevisiae and depends on the use of a different receptor repertoire. Understanding the role of cell wall recognition during infection might lead to understanding the boundaries between safety and pathogenicity.
在这项研究中，我们提供了证据表明，在人类单核细胞来源的树突状细胞中，暴露于致病性白念珠菌或非致病性酿酒酵母会引起不同的 Th17 反应。我们使用微生物的不同形式，如细胞、菌丝和孢子，作为工具包来剖析表面甘露聚糖在真菌免疫反应中的作用。与酿酒酵母酵母细胞诱导的 Th1 反应相反，用孢子或白念珠菌菌丝刺激的树突状细胞诱导细胞反应向 Th17 分化方向偏移。对特定甘露聚糖结构的差异识别是区分有害和无害真菌的主要调节因子。从孢子到酵母的转变对于酿酒酵母的共生至关重要，并且依赖于不同的受体谱的使用。了解感染过程中细胞壁识别的作用可能有助于理解安全性和致病性之间的界限。
