College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):347-350. doi: 10.1099/ijs.0.022400-0. Epub 2010 Mar 12.
A Gram-negative, neutrophilic and rod-shaped bacterium, strain ZH17(T), was isolated from a marine sediment of the East China Sea and subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0-7.5 % (w/v) NaCl and at pH 6.5-9.0; optimum growth was observed with 0.5-3.0 % (w/v) NaCl and at pH 7.5. Chemotaxonomic analysis showed ubiquinone-10 as predominant respiratory quinone and C(18 : 1)ω7c, 11-methyl C(18 : 1)ω7c, C(16 : 0), C(12 : 0) 3-OH and C(16 : 0) 2-OH as major fatty acids. The genomic DNA G+C content was 63.5 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Ruegeria. Strain ZH17(T) exhibited the closest phylogenetic affinity to the type strain of Ruegeria pomeroyi, with 97.2 % sequence similarity, and less than 97 % sequence similarity with respect to other described species of the genus Ruegeria. The DNA-DNA reassociation value between strain ZH17(T) and R. pomeroyi DSM 15171(T) was 50.7 %. On the basis of phenotypic and genotypic data, strain ZH17(T) represents a novel species of the genus Ruegeria, for which the name Ruegeria marina sp. nov. (type strain ZH17(T) =CGMCC 1.9108(T) =JCM 16262(T)) is proposed.
一株革兰氏阴性、嗜中性、杆状的细菌，菌株 ZH17(T)，从东海的海洋沉积物中分离出来，并进行了多相分类学鉴定。该菌在 0-7.5%（w/v）NaCl 存在下和 pH6.5-9.0 条件下生长；在 0.5-3.0%（w/v）NaCl 和 pH7.5 条件下生长最佳。化学分类分析表明，泛醌-10 为主要呼吸醌，C(18：1)ω7c、11-甲基 C(18：1)ω7c、C(16：0)、C(12：0) 3-OH 和 C(16：0) 2-OH 为主要脂肪酸。基因组 DNA G+C 含量为 63.5 mol%。比较 16S rRNA 基因序列分析表明，该菌株属于 Ruegeria 属。菌株 ZH17(T)与 Ruegeria pomeroyi 模式株的亲缘关系最近，序列相似性为 97.2%，与 Ruegeria 属的其他描述种的序列相似性小于 97%。菌株 ZH17(T)与 R. pomeroyi DSM 15171(T)之间的 DNA-DNA 同源性为 50.7%。基于表型和基因型数据，菌株 ZH17(T)代表 Ruegeria 属的一个新种，命名为 Ruegeria marina sp. nov.（模式菌株 ZH17(T) =CGMCC 1.9108(T) =JCM 16262(T)）。
