Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.
Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.
Int J Syst Evol Microbiol. 2020 May;70(5):3055-3061. doi: 10.1099/ijsem.0.004128.
A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterial strain, designated CAU 1488, was isolated from tidal flat sediment, and its taxonomic position was investigated using a polyphasic approach. The organism grew optimally at a temperature of 30 °C, at pH 7.0-7.5 and in the presence of 0-6 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1488 forms a lineage distinct from ZGT 118 (97.9 %), ZH17 (97.6 %), ITI 1157 (97.5 %), DSS-3 (97.1 %), ZGT108 (97.0 %), CC-GIMAT-2 (96.8 %), CECT 4292 (96.7 %) and J95 (95.9 %). Genome sequencing revealed that CAU 1488 had a genome size of 4.23 Mbp and a G+C content of 63.2 mol%. Overall genome related indexes including average nucleotide identity and digital DNA-DNA hybridization values were 75.0-83.0 % and 26.2 %, which are below the cutoffs of 95 and 70 %, respectively, indicating that strain CAU 1488 represents a distinct species from the members of the genus . The predominant quinone was ubiquinone-10 (Q-10). The major fatty acids were summed feature 8 (C ω7ω6; 60.7 %) and its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and unidentified aminolipids. On the basis of phenotypic, chemotaxonomic and genomic data, strain CAU 1488 constitutes a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAU 1488 (=KCTC 62996=NBRC 113693).
一株革兰氏阴性、需氧、不运动的杆状细菌菌株，被命名为 CAU 1488，从潮滩沉积物中分离得到，其分类地位通过多相研究进行了调查。该菌在 30°C、pH7.0-7.5 和 0-6%（w/v）NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CAU 1488 与 ZGT 118（97.9%）、ZH17（97.6%）、ITI 1157（97.5%）、DSS-3（97.1%）、ZGT108（97.0%）、CC-GIMAT-2（96.8%）、CECT 4292（96.7%）和 J95（95.9%）形成不同的谱系。基因组测序表明，CAU 1488 的基因组大小为 4.23 Mbp，G+C 含量为 63.2 mol%。包括平均核苷酸同一性和数字 DNA-DNA 杂交值在内的整体基因组相关指标分别为 75.0-83.0%和 26.2%，低于 95%和 70%的截止值，表明菌株 CAU 1488 代表了属内与成员不同的独特物种。主要醌类为泛醌-10（Q-10）。主要脂肪酸为 sumed feature 8（C ω7ω6；60.7%），其极性脂类由双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱和未鉴定的胺脂组成。基于表型、化学分类和基因组数据，菌株 CAU 1488 构成了属的一个新种，建议使用新种名称 sp. nov.。模式菌株为 CAU 1488（=KCTC 62996=NBRC 113693）。
