Department of Emergency Medicine, UCSF-Fresno Medical Center, 155 N. Fresno Drive, Fresno, CA 93701, USA.
J Med Toxicol. 2010 Mar;6(1):35-6. doi: 10.1007/s13181-010-0033-6.
Diquat is a bipyridyl herbicide with nephrotoxic effects. This in vitro study demonstrates a colorimetric test for detection of diquat in human urine. Urine specimens using ten concentrations of diquat herbicide solution and controls for urine and glyphosate were prepared. A two-step assay (addition of bicarbonate followed by sodium dithionite) was performed, with a resulting color change of the original solution for each specimen. Color change intensity was noted immediately and after 30 min, by gross visual inspection. A green color with concentration-dependent intensity was detected in all specimens, in which concentrations of diquat solution ranged from 0.73 to 730 mg/L. This colorimetric effect disappeared after 30 min. The sodium bicarbonate/dithionite test may be useful as a qualitative bedside technique for the detection of urinary diquat in the appropriate clinical setting.
敌草快是一种具有肾毒性的双吡啶类除草剂。本体外研究展示了一种用于检测人尿液中敌草快的比色试验方法。使用十种浓度的敌草快除草剂溶液和尿液及草甘膦对照品制备尿液标本。进行了两步法测定（先加入碳酸氢盐，再加入连二亚硫酸钠），每个标本的原始溶液均发生颜色变化。即刻及 30 分钟后通过肉眼观察记录颜色变化强度。所有标本均检测到具有浓度依赖性的绿色，敌草快溶液浓度范围为 0.73 至 730mg/L。30 分钟后这种比色效应消失。碳酸氢钠/连二亚硫酸钠试验可能可作为一种适用于特定临床环境的床边定性检测尿液中敌草快的技术。
