Central Laboratories for Frontier Technology, Kirin Holdings Co. Ltd., i-BIRD, Nonoichi, Ishikawa, Japan.
Planta. 2010 Jun;232(1):121-30. doi: 10.1007/s00425-010-1137-6. Epub 2010 Mar 13.
Ginger, Zingiber officinale Roscoe, contains a fragrant oil mainly composed of sesquiterpenes and monoterpenes. We isolated a cDNA that codes for a sesquiterpene synthase from young rhizomes of ginger, Z. officinale Roscoe, Japanese cultivar "Kintoki". The cDNA, designated ZoTps1, potentially encoded a protein that comprised 550 amino acid residues and exhibited 49-53% identity with those of the sesquiterpene synthases already isolated from the genus Zingiber. Recombinant Escherichia coli cells, in which ZoTps1 was coexpressed along with genes for D-mevalonate utilization, resulted in the production of a sesquiterpene (S)-beta-bisabolene exclusively with a D-mevalonolactone supplement. This result indicated that ZoTps1 was the (S)-beta-bisabolene synthase gene in ginger. ZoTPS1 was suggested to catalyze (S)-beta-bisabolene formation with the conversion of farnesyl diphosphate to nerolidyl diphosphate followed by the cyclization between position 1 and 6 carbons. The ZoTps1 transcript was detected in young rhizomes, but not in leaves, roots and mature rhizomes of the ginger "Kintoki".
生姜，姜属植物，含有一种主要由倍半萜烯和单萜烯组成的芳香油。我们从姜属植物日本品种“Kintoki”的幼根茎中分离出编码倍半萜烯合酶的 cDNA。该 cDNA 被命名为 ZoTps1，可能编码一种包含 550 个氨基酸残基的蛋白质，与已从姜属植物中分离出的倍半萜烯合酶具有 49-53%的同一性。共表达 ZoTps1 基因和 D-甲羟戊酸利用基因的重组大肠杆菌细胞，在补充 D-甲羟戊内酯的情况下，仅产生一种倍半萜烯（S）-β-法呢烯。这一结果表明 ZoTps1 是生姜中（S）-β-法呢烯合酶基因。ZoTPS1 可能通过法呢基二磷酸转化为香叶基二磷酸，然后在 1 位和 6 位碳原子之间环化，催化（S）-β-法呢烯的形成。在生姜“Kintoki”的幼根茎中检测到 ZoTps1 转录本，但在叶片、根和成熟根茎中未检测到。
