Guo Y, Zhang L, Xiao G, Cao S, Gu D, Tian W, Chen S
Institute of Genetics, Chinese Academy of Sciences, 100101, Beijing, China.
Sci China C Life Sci. 1997 Oct;40(5):496-501. doi: 10.1007/BF03183588.
Betaine as one of osmolytes plays an important role in osmoregulation of most high plants. Betaine aldehyde dehydrogenase (BADH) is the second enzyme involved in betaine biosynthesis. The BADH gene from a halophite,Atriplex hortensis, was transformed into rice cultivars by bombarment method. Totally 192 transgenic rice plants were obtained and most of them had higher salt tolerance than controls. Among transgenic plants transplanted in the saline pool containing 0.5% NaCl in a greenhouse, 22 survived. 13 of which set seeds, and the frequency of seed setting was very low, only 10%. But the controls could not grow under the same condition. The results of BADH activity assay and Northern blot showed that the BADH gene was integrated into chromosomes of transgenic plants and expressed.
甜菜碱作为一种渗透调节物质，在大多数高等植物的渗透调节中发挥着重要作用。甜菜碱醛脱氢酶（BADH）是参与甜菜碱生物合成的第二种酶。通过轰击法将来自盐生植物菠菜的BADH基因导入水稻品种。共获得192株转基因水稻植株，其中大部分植株的耐盐性高于对照。在温室中种植于含0.5% NaCl盐池中的转基因植株中，有22株存活。其中13株结实，结实率很低，仅为10%。但对照在相同条件下无法生长。BADH活性测定和Northern杂交结果表明，BADH基因已整合到转基因植物的染色体中并表达。
