Rostami Ali, Khazan Hooshang, Kazemi Bahram, Kia Eshrat Beigom, Bandepour Mojgan, Taghipour Niloofar, Mowlavi Gholamreza
Dept. of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dept. of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Public Health. 2017 Dec;46(12):1712-1719.
Trichinellosis is an important and neglected foodborne zoonotic infectious disease in worldwide. The most human outbreaks in recent years have been related to consumption of wild boar meat. This cross-sectional study determined the prevalence of spp. infections in hunted wild boars in northern Iran.
Thirty-five hunted wild boars were subjected in this study in 2015. All samples were examined by conventional artificial digestion method to detect of muscle larvae. Genomic DNA was extracted by phenol-chloroform method from isolated larvae. To identify the species, a PCR-based method was applied using the internal transcribed spacer 2 (ITS2) and mitochondrial small-subunit ribosomal RNA (rRNA) gene sequences.
The overall prevalence of spp. infection was 5.7% (2/35, 95%CI= 0-13.4). The mean larval burdens in two positive samples were 0.05 and 6 larvae per gr tissue muscle, respectively. The PCR reaction, using specific primers, yielded two 367 bp and 195 bp bands on agarose gel for ITS 2 and rrnS, respectively.
There is a hidden burden of spp. infection in wild boar population in Iran. Moreover, is the prevalent species circulating in wild boars of Iran. Therefore, education of the hunters and other consumers should be performed about the risk of consumption of raw or undercooked meat and meat products from wild boars.
旋毛虫病是一种在全球范围内重要且被忽视的食源性人畜共患传染病。近年来，大多数人类感染病例都与食用野猪肉有关。这项横断面研究确定了伊朗北部狩猎野猪中旋毛虫属物种的感染率。
2015年，本研究对35头狩猎野猪进行了检测。所有样本均采用传统人工消化法检测肌肉幼虫。从分离出的幼虫中采用酚氯仿法提取基因组DNA。为鉴定旋毛虫物种，应用基于聚合酶链反应（PCR）的方法，使用内部转录间隔区2（ITS2）和线粒体小亚基核糖体RNA（rRNA）基因序列。
旋毛虫属物种的总体感染率为5.7%（2/35，95%置信区间=0-13.4）。两个阳性样本中每克组织肌肉的幼虫平均负荷分别为0.05条和6条。使用特异性引物进行的PCR反应，在琼脂糖凝胶上分别针对ITS2和rrnS产生了两条367 bp和195 bp的条带。
伊朗野猪种群中存在旋毛虫属物种感染的潜在负担。此外，旋毛虫是伊朗野猪中流行的物种。因此，应针对猎人及其他消费者开展关于食用生的或未煮熟的野猪肉及肉制品风险的教育。
