National Institute of Research-Development for Microbiology and Immunology "Cantacuzino", 103 Splaiul Independenţei, 050096 Bucharest, Romania.
Exp Appl Acarol. 2010 Oct;52(2):193-7. doi: 10.1007/s10493-010-9353-0. Epub 2010 Mar 16.
We examined 200 questing Ixodes ricinus ticks (nymphs and adults) collected in three different sites in Sibiu County, Romania by polymerase chain reaction (PCR) followed by reverse line blot (RLB) for Borrelia burgdorferi s.l.. We detected the bacteria in 19% of the investigated ticks. The prevalence of infection was higher in nymphs (27%) than in adults (12%). Two B. burgdorferi sensu lato genospecies were detected: B. garinii (20%) and B. afzelii (80%). We did not detect any mixed infections in the investigated ticks. In two of the investigated sites B. burgdorferi prevalence values were comparable (25%), while in the third site the prevalence was lower ( approximately 7%). Our preliminary study provides evidence that Lyme disease spirochetes are present in various areas and at a relatively high prevalence in their vectors, thus posing a risk of infection to human subjects that undergo work or leisure activities in those areas.
我们通过聚合酶链反应（PCR）结合反向线印迹（RLB）法检测了在罗马尼亚锡比乌县三个不同地点采集的 200 只游离硬蜱（若虫和成虫），以检测伯氏疏螺旋体属。我们在 19%的调查蜱中检测到了细菌。感染的流行率在若虫（27%）中高于成虫（12%）。检测到两种伯氏疏螺旋体属：伯氏疏螺旋体（20%）和阿费尔伯氏疏螺旋体（80%）。我们没有发现调查蜱中有任何混合感染。在所调查的两个地点，伯氏疏螺旋体的流行率相当（25%），而在第三个地点，流行率较低（约 7%）。我们的初步研究表明，莱姆病螺旋体存在于不同地区，且在其传播媒介中存在较高的流行率，因此在这些地区从事工作或休闲活动的人类可能面临感染风险。
