Kalmár Zsuzsa, Dumitrache Mirabela Oana, D'Amico Gianluca, Matei Ioana Adriana, Ionică Angela Monica, Gherman Călin Mircea, Lupșe Mihaela, Mihalca Andrei Daniel
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania.
Department of Microbiology, Immunology and Epidemiology, Faculty of Veterinary Medicine, Calea Mănăştur, no. 3-5, 400372 Cluj-Napoca, Romania.
Pathogens. 2020 May 19;9(5):390. doi: 10.3390/pathogens9050390.
Ticks are medically important vectors of infectious diseases that are able to transmit pathogens to humans and animals. Tick-borne diseases represent a major health concern, posing an increasing risk to the public health during the last century and affecting millions of people. The aim of the current study was to provide epidemiological data regarding the presence of certain tick-borne pathogens in ticks feeding on humans in Romania. Overall, 522 ticks collected from humans were screened for six pathogens: spp., , spp., spp., spp., and . Ticks attached to humans were collected between 2013-2015 in Cluj County, Romania. Conventional, nested and quantitative PCR were used to detect specific genetic sequences of each pathogen. For identifying the infectious agents, positive samples were sequenced. The infection prevalence was 21.07% from which 8.18% were mixed infections. The detected agents were spp., and spp. The present data reveal the endemic occurrence of potentially zoonotic pathogens in Romania. Revealing the current distribution of tick-borne pathogens in ticks collected from humans may provide new insights in understanding the complex ecology of tick-borne diseases and enlightens current knowledge about the infection prevalence at local, regional and national levels.
蜱是具有重要医学意义的传染病传播媒介，能够将病原体传播给人类和动物。蜱传疾病是一个主要的健康问题，在上个世纪对公众健康构成了日益增加的风险，影响了数百万人。本研究的目的是提供有关罗马尼亚以人类为宿主的蜱中某些蜱传病原体存在情况的流行病学数据。总体而言，对从人类身上采集的522只蜱进行了六种病原体的筛查：[具体病原体名称1]、[具体病原体名称2]、[具体病原体名称3]、[具体病原体名称4]、[具体病原体名称5]和[具体病原体名称6]。附着在人类身上的蜱于2013年至2015年期间在罗马尼亚克卢日县采集。采用常规、巢式和定量PCR检测每种病原体的特定基因序列。为了鉴定感染因子，对阳性样本进行了测序。感染率为21.07%，其中8.18%为混合感染。检测到的病原体为[具体病原体名称1]、[具体病原体名称2]和[具体病原体名称3]。目前的数据揭示了罗马尼亚潜在人畜共患病原体的地方流行情况。揭示从人类身上采集的蜱中蜱传病原体的当前分布情况，可能为理解蜱传疾病的复杂生态学提供新的见解，并增进对地方、区域和国家层面感染率的现有认识。
