LaFleur Rhonda L, Callister Steven M, Dant Jennifer C, Jobe Dean A, Lovrich Steven D, Warner Thomas F, Wasmoen Terri L, Schell Ronald F
Intervet/Schering-Plough Animal Health Corporation, 21401 W. Center Road, Elkhorn, NE 68022-2202, USA.
Clin Vaccine Immunol. 2010 May;17(5):870-4. doi: 10.1128/CVI.00524-09. Epub 2010 Mar 17.
Laboratory-reared beagles were vaccinated with a placebo or a bacterin comprised of Borrelia burgdorferi S-1-10 and ospA-negative/ospB-negative B. burgdorferi 50772 and challenged after 1 year with B. burgdorferi-infected Ixodes scapularis ticks. For the placebo recipients, spirochetes were recovered from 9 (60%) skin biopsy specimens collected after 1 month, and the organisms persisted in the skin thereafter. Ten (67%) dogs also developed joint infection (3 dogs), lameness or synovitis (7 dogs), or B. burgdorferi-specific antibodies (8 dogs). For the vaccine recipients, spirochetes were recovered from 6 (40%) skin biopsy specimens collected after 1 month. However, subsequent biopsy specimens were negative, and the dogs failed to develop joint infection (P = 0.224), lameness/synovitis (P = 0.006), or Lyme disease-specific antibody responses (P = 0.002). The bacterin provided a high level of protection for 1 year after immunization, and the addition of the OspC-producing B. burgdorferi 50772 provided enhanced protection.
将实验室饲养的比格犬用安慰剂或由伯氏疏螺旋体S-1-10和ospA阴性/ospB阴性的伯氏疏螺旋体50772组成的菌苗进行接种，并在1年后用感染了伯氏疏螺旋体的肩突硬蜱进行攻击。对于接受安慰剂的犬只，在1个月后采集的9份（60%）皮肤活检标本中检测到螺旋体，此后这些微生物在皮肤中持续存在。10只（67%）犬还发生了关节感染（3只犬）、跛行或滑膜炎（7只犬）或产生了伯氏疏螺旋体特异性抗体（8只犬）。对于接种疫苗的犬只，在1个月后采集的6份（40%）皮肤活检标本中检测到螺旋体。然而，随后的活检标本为阴性，且这些犬未发生关节感染（P = 0.224）、跛行/滑膜炎（P = 0.006）或莱姆病特异性抗体反应（P = 0.002）。该菌苗在免疫后1年内提供了高水平的保护，并且添加产生OspC的伯氏疏螺旋体50772可增强保护作用。
