LaFleur Rhonda L, Dant Jennifer C, Wasmoen Terri L, Callister Steven M, Jobe Dean A, Lovrich Steven D, Warner Thomas F, Abdelmagid O, Schell Ronald F
Schering Plough Animal Health Corporation, Elkhorn, Nebraska 68022-2202, USA.
Clin Vaccine Immunol. 2009 Feb;16(2):253-9. doi: 10.1128/CVI.00373-08. Epub 2008 Dec 3.
Groups of 15 laboratory-bred beagles were vaccinated and boosted with either a placebo or adjuvanted bivalent bacterin comprised of a traditional Borrelia burgdorferi strain and a unique ospA- and ospB-negative B. burgdorferi strain that expressed high levels of OspC and then challenged with B. burgdorferi-infected Ixodes scapularis ticks. The vaccinated dogs produced high titers of anti-OspA and anti-OspC borreliacidal antibodies, including borreliacidal antibodies specific for an epitope within the last seven amino acids at the OspC carboxy terminus (termed OspC7) that was conserved among pathogenic Borrelia genospecies. In addition, spirochetes were eliminated from the infected ticks that fed on the bacterin recipients, B. burgdorferi was not isolated from the skin or joints, and antibody responses associated specifically with canine infection with B. burgdorferi were not produced. In contrast, B. burgdorferi was recovered from engorged ticks that fed on 13 (87%) placebo-vaccinated dogs (P<0.0001), skin biopsy specimens from 14 (93%) dogs (P<0.0001), and joint tissue specimens from 8 (53%) dogs (P=0.0022). In addition, 14 (93%) dogs developed specific antibody responses against B. burgdorferi proteins, including 11 (73%) with C6 peptide antibodies (P<0.0001). Moreover, 10 (67%) dogs developed Lyme disease-associated joint abnormalities (P<0.0001), including 4 (27%) dogs that developed joint stiffness or lameness and 6 (40%) that developed chronic joint inflammation (synovitis). The results therefore confirmed that the bacterin provided a high level of protection against Lyme disease shortly after immunization.
将15只实验室培育的小猎犬分成几组，分别用安慰剂或由传统的伯氏疏螺旋体菌株和一种独特的ospA和ospB阴性、表达高水平OspC的伯氏疏螺旋体菌株组成的二价佐剂菌苗进行接种和加强免疫，然后用感染了伯氏疏螺旋体的肩突硬蜱进行攻击。接种疫苗的犬产生了高滴度的抗OspA和抗OspC杀螺旋体抗体，包括对OspC羧基末端最后七个氨基酸内一个表位特异的杀螺旋体抗体（称为OspC7），该表位在致病性伯氏疏螺旋体基因种中是保守的。此外，从以菌苗接种犬为宿主的感染蜱中清除了螺旋体，在皮肤或关节中未分离到伯氏疏螺旋体，也未产生与犬感染伯氏疏螺旋体特异性相关的抗体反应。相比之下，从以13只（87%）接种安慰剂的犬为宿主的饱血蜱中（P<0.0001）、14只（93%）犬的皮肤活检标本中（P<0.0001）以及8只（53%）犬的关节组织标本中（P=0.0022）分离到了伯氏疏螺旋体。此外，14只（93%）犬产生了针对伯氏疏螺旋体蛋白的特异性抗体反应，包括11只（73%）产生C6肽抗体的犬（P<0.0001）。而且，10只（67%）犬出现了莱姆病相关的关节异常（P<0.0001），包括4只（27%）出现关节僵硬或跛行的犬和6只（40%）出现慢性关节炎症（滑膜炎）的犬。因此，结果证实该菌苗在免疫后不久就提供了高水平的抗莱姆病保护。
