丝状真菌的原生质体转化

Protoplast transformation of filamentous fungi.

作者信息

Turgeon B Gillian, Condon Bradford, Liu Jinyuan, Zhang Ning

机构信息

Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, USA.

出版信息

Methods Mol Biol. 2010;638:3-19. doi: 10.1007/978-1-60761-611-5_1.

DOI:10.1007/978-1-60761-611-5_1

PMID:20238257

Abstract

The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

摘要

详细描述了用于丝状真菌转化的原生质体方法，以及用于将标记突变引入真菌基因组的限制酶介导整合（REMI）程序。还详细介绍了一种用于产生PCR片段以进行目标基因整合和缺失的分裂标记方法。