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一种形成J聚集体的亲脂性阳离子JC-1揭示的线粒体膜电位中的细胞内异质性

Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.

作者信息

Smiley S T, Reers M, Mottola-Hartshorn C, Lin M, Chen A, Smith T W, Steele G D, Chen L B

机构信息

Dana-Farber Cancer Institute, Boston, MA.

出版信息

Proc Natl Acad Sci U S A. 1991 May 1;88(9):3671-5. doi: 10.1073/pnas.88.9.3671.

DOI:10.1073/pnas.88.9.3671
PMID:2023917
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC51514/
Abstract

By using a potential-dependent J-aggregate-forming delocalized lipophilic cation, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine++ + iodide (JC-1), we find that membrane potentials across mitochondria in a living cell can be heterogeneous. Remarkably, even within a long contiguous mitochondrion, regional heterogeneity in membrane potentials appears to be possible.

摘要

通过使用一种电位依赖性J-聚集体形成的离域亲脂性阳离子，5,5',6,6'-四氯-1,1',3,3'-四乙基苯并咪唑碳菁碘化物（JC-1），我们发现活细胞中线粒体的跨膜电位可能是异质性的。值得注意的是，即使在一个长的连续线粒体中，膜电位的区域异质性似乎也是可能的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/3d69e1bfffb0/pnas01059-0174-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/950cbd889188/pnas01059-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/3c937638e874/pnas01059-0172-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/f8b97b83be25/pnas01059-0172-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/8c3eb81b9aa1/pnas01059-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/133936b0bbec/pnas01059-0173-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/410f0c2424e1/pnas01059-0173-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/1ae63de573c0/pnas01059-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/394b73b903a8/pnas01059-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/3d69e1bfffb0/pnas01059-0174-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/950cbd889188/pnas01059-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/3c937638e874/pnas01059-0172-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/f8b97b83be25/pnas01059-0172-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/8c3eb81b9aa1/pnas01059-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/133936b0bbec/pnas01059-0173-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/410f0c2424e1/pnas01059-0173-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/1ae63de573c0/pnas01059-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/394b73b903a8/pnas01059-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab1/51514/3d69e1bfffb0/pnas01059-0174-c.jpg

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