Schipperus M R, Sonneveld P, Lindemans J, van Lom K, Vlastuin M, Abels J
Department of Haematology, University Hospital, The Netherlands.
Br J Haematol. 1991 Apr;77(4):515-22. doi: 10.1111/j.1365-2141.1991.tb08619.x.
Interleukin-1 (Il-1) and Interleukin-6 (Il-6) have been reported to enhance the growth factor dependent colony formation of normal primitive haematopoietic progenitor cells as well as of leukaemic blast-cell progenitors. We investigated the effects of Il-1 beta and Il-6 in combination with granulocyte-macrophage colony-stimulating factor (GM-CSF) on the in vitro colony formation of myeloid progenitors from 23 patients with a myelodysplastic syndrome (MDS). Neither Il-1 beta nor Il-6 were found to have colony stimulating activity on their own. In normal bone marrow cultures, either stimulated with optimal or suboptimal doses of GM-CSF, no enhancing or antagonistic effect of Il-6 or Il-1 beta was detected. In a majority of the MDS cases, however, an enhancing effect of Il-6 and Il-1 beta in combination with GM-CSF was observed (20/23 and 10/21 cases respectively). In three cases of the Il-6 and GM-CSF combination an antagonistic effect was observed as well as in four cases of the Il-1 beta and GM-CSF combination. A delayed addition of Il-6 to the cultures did not result in an abrogation of the effect, indicating that Il-6 is not required immediately at the initiation of the culture. These results indicate that costimulation with Il-6 or Il-1 beta is able to augment the GM-CSF activity on MDS myeloid progenitor cells.
据报道，白细胞介素-1（Il-1）和白细胞介素-6（Il-6）可增强正常原始造血祖细胞以及白血病母细胞祖细胞的生长因子依赖性集落形成。我们研究了Il-1β和Il-6与粒细胞-巨噬细胞集落刺激因子（GM-CSF）联合应用对23例骨髓增生异常综合征（MDS）患者髓系祖细胞体外集落形成的影响。未发现Il-1β和Il-6单独具有集落刺激活性。在正常骨髓培养物中，无论用最佳剂量还是次最佳剂量的GM-CSF刺激，均未检测到Il-6或Il-1β的增强或拮抗作用。然而，在大多数MDS病例中，观察到Il-6和Il-1β与GM-CSF联合应用具有增强作用（分别为20/23例和10/21例）。在3例Il-6与GM-CSF联合应用的病例以及4例Il-1β与GM-CSF联合应用的病例中观察到拮抗作用。在培养物中延迟添加Il-6并未导致该效应消失，这表明在培养开始时并不立即需要Il-6。这些结果表明，Il-6或Il-1β的共刺激能够增强GM-CSF对MDS髓系祖细胞的活性。
