Copty Anan B, Neve-Oz Yair, Barak Itai, Golosovsky Michael, Davidov Dan
The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, Israel.
Biophys J. 2006 Aug 15;91(4):1413-23. doi: 10.1529/biophysj.106.084111. Epub 2006 May 26.
We have compared the effect of microwave irradiation and of conventional heating on the fluorescence of solution-based green fluorescent protein. A specialized near-field 8.5 GHz microwave applicator operating at 250 mW input microwave power was used. The solution temperature, the intensity, and the spectrum of the green fluorescent protein fluorescence 1), under microwave irradiation and 2), under conventional heating, were measured. In both cases the fluorescence intensity decreases and the spectrum becomes red-shifted. Although the microwave irradiation heats the solution, the microwave-induced changes in fluorescence cannot be explained by heating alone. Several possible scenarios are discussed.
我们比较了微波辐射和传统加热对基于溶液的绿色荧光蛋白荧光的影响。使用了一个专门的近场8.5 GHz微波施加器，其输入微波功率为250 mW。测量了1）在微波辐射下和2）在传统加热下绿色荧光蛋白荧光的溶液温度、强度和光谱。在这两种情况下，荧光强度均降低，光谱发生红移。尽管微波辐射会加热溶液，但微波引起的荧光变化不能仅用加热来解释。讨论了几种可能的情况。
