Shoji J, Kato T, Hinoo H
J Antibiot (Tokyo). 1977 Dec;30(12):1035-41. doi: 10.7164/antibiotics.30.1035.
Amino acid analysis on the acid hydrolyzate of polymyxin S1 revealed its amino acid composition. Isolation of the constituent amino acids and measurement of their optical activities clarified their chiralities: Dab(5L), Thr(3L), Ser(1D) and Phe(1D). The constituent fatty acid was identified with anteisononanoic acid by gas chromatography and mass spectrometry. By the action of polymyxin acylase, deacyl polymyxin S was obtained. Successive EDMAN degradation reaction on deacyl polymyxin S revealed the amino acid sequence. Further evidence for the structure of polymyxin S1 was obtained by partial acid hydrolysis on tetra-(DNP)-polymyxin S1.
对多粘菌素S1的酸水解产物进行氨基酸分析，揭示了其氨基酸组成。分离组成氨基酸并测量其旋光活性，明确了它们的手性：二氨基丁酸（5L）、苏氨酸（3L）、丝氨酸（1D）和苯丙氨酸（1D）。通过气相色谱和质谱法将组成脂肪酸鉴定为异壬酸。通过多粘菌素酰基转移酶的作用，得到了脱酰基多粘菌素S。对脱酰基多粘菌素S进行连续的埃德曼降解反应，揭示了氨基酸序列。通过对四 - （二硝基苯基） - 多粘菌素S1进行部分酸水解，获得了多粘菌素S1结构的进一步证据。
