Chamley L W, McKay E J, Pattison N S
Department of Obstetrics and Gynaecology, University of Auckland, New Zealand.
Thromb Res. 1991 Feb 1;61(3):291-9. doi: 10.1016/0049-3848(91)90106-7.
Two distinct types of anticardiolipin antibodies are described, one of which requires the presence of a serum factor (cofactor) to bind cardiolipin in ELISA and liposome affinity systems. The second type does not require this cofactor. The requirement of a cofactor for the binding of some but not all anticardiolipin antibodies provides an explanation for the confounding variability of the results of assays for these antibodies. It also explains why blocking agents such as BSA and gelatin do not produce consistent results in this assay.
已描述了两种不同类型的抗心磷脂抗体，其中一种在酶联免疫吸附测定（ELISA）和脂质体亲和系统中需要血清因子（辅助因子）的存在才能结合心磷脂。第二种类型则不需要这种辅助因子。部分而非全部抗心磷脂抗体结合需要辅助因子，这就解释了这些抗体检测结果令人困惑的变异性。这也解释了为什么诸如牛血清白蛋白（BSA）和明胶等封闭剂在此检测中不能产生一致的结果。
