• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Renaturation of complementary single-stranded DNA circles: complete rewinding facilitated by the DNA untwisting enzyme.互补单链DNA环的复性：DNA解旋酶促进完全重绕
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5328-32. doi: 10.1073/pnas.74.12.5328.
2
Escherichia coli DNA topoisomerase I catalyzed linking of single-stranded rings of complementary base sequences.大肠杆菌DNA拓扑异构酶I催化互补碱基序列单链环的连接。
Nucleic Acids Res. 1978 Oct;5(10):3811-20. doi: 10.1093/nar/5.10.3811.
3
Interaction of the DNA untwisting enzyme with the SV40 nucleoprotein complex.DNA解旋酶与SV40核蛋白复合体的相互作用。
Nucleic Acids Res. 1978 Feb;5(2):623-35. doi: 10.1093/nar/5.2.623.
4
Ethidium bromide-mediated renaturation of denatured closed circular DNAs. The nature of denaturation-resistant fractions of bacteriophage PM2 closed circular DNA.溴化乙锭介导的变性闭环DNA复性。噬菌体PM2闭环DNA抗变性组分的性质。
Nucleic Acids Res. 1980 Feb 11;8(3):673-701. doi: 10.1093/nar/8.3.673.
5
Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.DNA解旋酶造成的链断裂导致该酶与DNA共价连接。
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3800-4. doi: 10.1073/pnas.74.9.3800.
6
Type I DNA topoisomerases from mammalian cell nuclei interlock strains and promote renaturation of denatured closed circular PM2 DNA.来自哺乳动物细胞核的I型DNA拓扑异构酶使链相互交锁，并促进变性的闭环PM2 DNA复性。
Biochim Biophys Acta. 1981 Sep 28;655(2):199-209. doi: 10.1016/0005-2787(81)90010-1.
7
Action of S1 nuclease on nicked circular simian virus 40 DNA.S1核酸酶对带切口的环状猴病毒40 DNA的作用。
Biochem Biophys Res Commun. 1975 May 19;64(2):709-16. doi: 10.1016/0006-291x(75)90378-2.
8
Type II DNA topoisomerases: enzymes that can unknot a topologically knotted DNA molecule via a reversible double-strand break.II型DNA拓扑异构酶：能够通过可逆的双链断裂解开拓扑学上打结的DNA分子的酶。
Cell. 1980 Mar;19(3):697-707. doi: 10.1016/s0092-8674(80)80046-8.
9
The role of single-strand breaks in the catenation reaction catalyzed by the rat type I topoisomerase.单链断裂在大鼠I型拓扑异构酶催化的连环反应中的作用。
J Biol Chem. 1986 Apr 15;261(11):5130-7.
10
The beta protein of phage lambda binds preferentially to an intermediate in DNA renaturation.噬菌体λ的β蛋白优先结合于DNA复性过程中的一个中间体。
J Mol Biol. 1998 Mar 6;276(4):721-31. doi: 10.1006/jmbi.1997.1573.

引用本文的文献

1
Construction of DNA hemicatenanes from two small circular DNA molecules.由两个小的环状DNA分子构建DNA半连环体。
PLoS One. 2015 Mar 23;10(3):e0119368. doi: 10.1371/journal.pone.0119368. eCollection 2015.
2
DNA topoisomerases from rat liver: physiological variations.大鼠肝脏中的DNA拓扑异构酶：生理变异
Nucleic Acids Res. 1983 Feb 25;11(4):1059-75. doi: 10.1093/nar/11.4.1059.
3
DNA breakage and closure by rat liver type 1 topoisomerase: separation of the half-reactions by using a single-stranded DNA substrate.
Proc Natl Acad Sci U S A. 1981 May;78(5):2883-7. doi: 10.1073/pnas.78.5.2883.
4
Catenation and knotting of duplex DNA by type 1 topoisomerases: a mechanistic parallel with type 2 topoisomerases.1型拓扑异构酶对双链DNA的连环化和打结：与2型拓扑异构酶的机制平行关系
Proc Natl Acad Sci U S A. 1981 Feb;78(2):843-7. doi: 10.1073/pnas.78.2.843.
5
Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.大鼠肝脏切口-封闭酶对单链DNA的断裂及DNA-酶复合物的形成
Nucleic Acids Res. 1980 Dec 20;8(24):6129-42. doi: 10.1093/nar/8.24.6129.
6
Evidence for inclusion of regions of nonhomology in heteroduplex products of bacteriophage lambda recombination.噬菌体λ重组异源双链产物中包含非同源区域的证据。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7180-4. doi: 10.1073/pnas.81.22.7180.
7
Double Holliday structure: a possible in vivo intermediate form of general recombination in Escherichia coli.双Holliday结构：大肠杆菌中一般重组可能的体内中间形式。
Mol Gen Genet. 1983;191(2):213-20. doi: 10.1007/BF00334816.
8
Plasmid recombination intermediates generated in a Saccharomyces cerevisiae cell-free recombination system.在酿酒酵母无细胞重组系统中产生的质粒重组中间体。
Mol Cell Biol. 1985 Sep;5(9):2361-8. doi: 10.1128/mcb.5.9.2361-2368.1985.
9
Topoisomerase I phosphorylation in vitro and in rapidly growing Novikoff hepatoma cells.
EMBO J. 1985 Nov;4(11):2921-6. doi: 10.1002/j.1460-2075.1985.tb04024.x.
10
Biochemical assay designed to detect formation of recombination intermediates in vitro.
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1084-8. doi: 10.1073/pnas.76.3.1084.

本文引用的文献

1
THE CYCLIC HELIX AND CYCLIC COIL FORMS OF POLYOMA VIRAL DNA.多瘤病毒DNA的环状螺旋和环状盘绕形式
Proc Natl Acad Sci U S A. 1963 Oct;50(4):730-8. doi: 10.1073/pnas.50.4.730.
2
EVIDENCE FOR A RING STRUCTURE OF POLYOMA VIRUS DNA.多瘤病毒DNA环状结构的证据。
Proc Natl Acad Sci U S A. 1963 Aug;50(2):236-43. doi: 10.1073/pnas.50.2.236.
3
Effects of the conformation of single-stranded DNA on renaturation and aggregation.单链DNA构象对复性和聚集的影响。
J Mol Biol. 1969 Apr;41(2):199-209. doi: 10.1016/0022-2836(69)90385-4.
4
Kinetics of renaturation of DNA.DNA复性动力学
J Mol Biol. 1968 Feb 14;31(3):349-70. doi: 10.1016/0022-2836(68)90414-2.
5
T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.T4噬菌体基因32：DNA复制与重组中的一种结构蛋白。
Nature. 1970 Sep 26;227(5265):1313-8. doi: 10.1038/2271313a0.
6
Models of genetic recombination.基因重组模型。
Annu Rev Microbiol. 1974;28(0):445-68. doi: 10.1146/annurev.mi.28.100174.002305.
7
Rec-mediated recombinational hot spot activity in bacteriophage lambda. I. Hot spot activity associated with spi-deletions and bio substitutions.噬菌体λ中Rec介导的重组热点活性。I. 与spi缺失和bio取代相关的热点活性。
Genetics. 1974 Jul;77(3):409-23. doi: 10.1093/genetics/77.3.409.
8
Dependence of the sedimentation coefficient of denatured closed circular DNA in alkali on the degree of strand interwinding. The absolute sense of supercoils.变性闭环DNA在碱中的沉降系数对链间缠绕程度的依赖性。超螺旋的绝对意义。
J Mol Biol. 1974 Feb 15;83(1):35-45. doi: 10.1016/0022-2836(74)90422-7.
9
A buoyant method for the determination of the superhelix density of closed circular DNA.一种用于测定闭环DNA超螺旋密度的浮力方法。
J Mol Biol. 1971 Nov 28;62(1):1-19. doi: 10.1016/0022-2836(71)90127-6.
10
An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).一种来自哺乳动物细胞的能解开超螺旋DNA的活性物质——一种可能用于DNA复制的旋转酶（多瘤病毒-溴化乙锭-小鼠胚胎细胞-染料结合测定）
Proc Natl Acad Sci U S A. 1972 Jan;69(1):143-6. doi: 10.1073/pnas.69.1.143.

互补单链DNA环的复性：DNA解旋酶促进完全重绕

Renaturation of complementary single-stranded DNA circles: complete rewinding facilitated by the DNA untwisting enzyme.

作者信息

Champoux J J

出版信息

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5328-32. doi: 10.1073/pnas.74.12.5328.

DOI:10.1073/pnas.74.12.5328
PMID:202951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431704/
Abstract

Renaturation of two complementary single-stranded circles should be limited by topological constraints against the rewinding of the DNA helix. If a mixture of complementary single-stranded rings is annealed and then treated with the DNA untwisting enzyme, the DNA circles completely renature as judged by (i) the presence of interlocked rings that sediment at 53 S in alkali, (ii) the buoyant density of the renatured DNA in CsCl gradients containing ethidium bromide, and (iii) the resistance of the product to the single-strand-specific S1 nuclease. Therefore, the DNA untwisting enzyme is able to provide a transient single-strand break that is sufficient to allow the two strands to completely rewind. The possibility that the untwisting enzyme might facilitate the initiation of the process of genetic recombination is discussed.

摘要

两条互补单链环的复性应受到DNA螺旋重绕拓扑限制的制约。如果将互补单链环的混合物退火，然后用DNA解旋酶处理，通过以下几点判断，DNA环会完全复性：（i）存在在碱性条件下以53 S沉降的互锁环；（ii）在含有溴化乙锭的CsCl梯度中复性DNA的浮力密度；（iii）产物对单链特异性S1核酸酶的抗性。因此，DNA解旋酶能够提供一个瞬时单链断裂，足以使两条链完全重绕。文中讨论了解旋酶可能促进基因重组过程起始的可能性。