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互补单链DNA环的复性：DNA解旋酶促进完全重绕

Renaturation of complementary single-stranded DNA circles: complete rewinding facilitated by the DNA untwisting enzyme.

作者信息

Champoux J J

出版信息

Proc Natl Acad Sci U S A. 1977 Dec;74(12):5328-32. doi: 10.1073/pnas.74.12.5328.

DOI:10.1073/pnas.74.12.5328
PMID:202951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431704/
Abstract

Renaturation of two complementary single-stranded circles should be limited by topological constraints against the rewinding of the DNA helix. If a mixture of complementary single-stranded rings is annealed and then treated with the DNA untwisting enzyme, the DNA circles completely renature as judged by (i) the presence of interlocked rings that sediment at 53 S in alkali, (ii) the buoyant density of the renatured DNA in CsCl gradients containing ethidium bromide, and (iii) the resistance of the product to the single-strand-specific S1 nuclease. Therefore, the DNA untwisting enzyme is able to provide a transient single-strand break that is sufficient to allow the two strands to completely rewind. The possibility that the untwisting enzyme might facilitate the initiation of the process of genetic recombination is discussed.

摘要

两条互补单链环的复性应受到DNA螺旋重绕拓扑限制的制约。如果将互补单链环的混合物退火，然后用DNA解旋酶处理，通过以下几点判断，DNA环会完全复性：（i）存在在碱性条件下以53 S沉降的互锁环；（ii）在含有溴化乙锭的CsCl梯度中复性DNA的浮力密度；（iii）产物对单链特异性S1核酸酶的抗性。因此，DNA解旋酶能够提供一个瞬时单链断裂，足以使两条链完全重绕。文中讨论了解旋酶可能促进基因重组过程起始的可能性。

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Renaturation of complementary single-stranded DNA circles: complete rewinding facilitated by the DNA untwisting enzyme.互补单链DNA环的复性：DNA解旋酶促进完全重绕
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5328-32. doi: 10.1073/pnas.74.12.5328.
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本文引用的文献

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THE CYCLIC HELIX AND CYCLIC COIL FORMS OF POLYOMA VIRAL DNA.多瘤病毒DNA的环状螺旋和环状盘绕形式
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Rec-mediated recombinational hot spot activity in bacteriophage lambda. I. Hot spot activity associated with spi-deletions and bio substitutions.噬菌体λ中Rec介导的重组热点活性。I. 与spi缺失和bio取代相关的热点活性。
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Dependence of the sedimentation coefficient of denatured closed circular DNA in alkali on the degree of strand interwinding. The absolute sense of supercoils.变性闭环DNA在碱中的沉降系数对链间缠绕程度的依赖性。超螺旋的绝对意义。
J Mol Biol. 1974 Feb 15;83(1):35-45. doi: 10.1016/0022-2836(74)90422-7.
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A buoyant method for the determination of the superhelix density of closed circular DNA.一种用于测定闭环DNA超螺旋密度的浮力方法。
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An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).一种来自哺乳动物细胞的能解开超螺旋DNA的活性物质——一种可能用于DNA复制的旋转酶（多瘤病毒-溴化乙锭-小鼠胚胎细胞-染料结合测定）
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