DNA解旋酶与SV40核蛋白复合体的相互作用。
Interaction of the DNA untwisting enzyme with the SV40 nucleoprotein complex.
作者信息
Young L S, Champoux J J
出版信息
Nucleic Acids Res. 1978 Feb;5(2):623-35. doi: 10.1093/nar/5.2.623.
Abstract
The SV40 nucleoprotein complex which was isolated from infected CV-1 cells did not possess an active DNA untwisting enzyme. The superhelix density of the DNA in the chromatin complex was unchanged after treatment with purified rat liver DNA untwisting enzyme. However, in the presence of ethidium bromide (1 microgram/ml) the superhelix density was changed. Moreover, the nicked intermediate in the DNA untwisting reaction could be detected using the chromatin DNA as a substrate. These results show that the DNA in the SV40 chromatin which is accessible to the DNA untwisting enzyme is under no topological strain.
摘要
从感染的CV - 1细胞中分离出的SV40核蛋白复合物不具备活性DNA解旋酶。用纯化的大鼠肝脏DNA解旋酶处理后，染色质复合物中DNA的超螺旋密度未发生变化。然而，在溴化乙锭（1微克/毫升）存在的情况下，超螺旋密度发生了改变。此外，以染色质DNA为底物时，可检测到DNA解旋反应中的切口中间体。这些结果表明，SV40染色质中可被DNA解旋酶作用的DNA不存在拓扑学张力。
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本文引用的文献
1
Chromatin structure: deduced from a minichromosome.染色质结构：从小染色体推断出来的。
Science. 1975 Mar 28;187(4182):1202-3. doi: 10.1126/science.187.4182.1202.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
THE CYCLIC HELIX AND CYCLIC COIL FORMS OF POLYOMA VIRAL DNA.多瘤病毒DNA的环状螺旋和环状盘绕形式
Proc Natl Acad Sci U S A. 1963 Oct;50(4):730-8. doi: 10.1073/pnas.50.4.730.
4
EVIDENCE FOR A RING STRUCTURE OF POLYOMA VIRUS DNA.多瘤病毒DNA环状结构的证据。
Proc Natl Acad Sci U S A. 1963 Aug;50(2):236-43. doi: 10.1073/pnas.50.2.236.
5
General model for the chromosomes of higher organisms.高等生物染色体的通用模型。
Nature. 1971 Nov 5;234(5323):25-7. doi: 10.1038/234025a0.
6
Interaction between DNA and an Escherichia coli protein omega.DNA与大肠杆菌蛋白ω之间的相互作用。
J Mol Biol. 1971 Feb 14;55(3):523-33. doi: 10.1016/0022-2836(71)90334-2.
7
Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.染色质亚结构。一种核脱氧核糖核酸酶对染色质DNA在规则间隔位点的消化作用。
Biochem Biophys Res Commun. 1973 May 15;52(2):504-10. doi: 10.1016/0006-291x(73)90740-7.
8
The properties and origin of the proteins in the SV40 nucleoprotein complex.猴空泡病毒40核蛋白复合体中蛋白质的性质与起源
Virology. 1974 Sep;61(1):11-21. doi: 10.1016/0042-6822(74)90237-2.
9
SV40 nucleoprotein complex activity unwinds superhelical turns in SV40 DNA.SV40核蛋白复合体活性解开SV40 DNA中的超螺旋圈。
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J Virol. 1973 Oct;12(4):901-8. doi: 10.1128/JVI.12.4.901-908.1973.
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