Department of Experimental Phylaxiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto-city, Kumamoto 860-8556, Japan.
Comp Immunol Microbiol Infect Dis. 2010 Dec;33(6):e81-8. doi: 10.1016/j.cimid.2010.02.006. Epub 2010 Mar 17.
In this study, we infected NOD/Scid/Jak3null mice engrafted human peripheral blood leukocytes (hu-PBL-NOJ) with measles virus Edmonston B strain (MV-Edm) expressing hepatitis C virus (HCV) envelope proteins (rMV-E1E2) to evaluate the immunogenicity as a vaccine candidate. Although human leukocytes could be isolated from the spleen of mock-infected mice during the 2-weeks experiment, the proportion of engrafted human leukocytes in mice infected with MV (10(3)-10(5)pfu) or rMV-E1E2 (10(4)pfu) was decreased. Viral infection of the splenocytes was confirmed by the development of cytopathic effects (CPEs) in co-cultures of splenocytes and B95a cells and verified using RT-PCR. Finally, human antibodies against MV were more frequently observed than E2-specific antibodies in serum from mice infected with a low dose of virus (MV, 10(0)-10(1)pfu, and rMV-E1E2, 10(1)-10(2)pfu). These results showed the possibility of hu-PBL-NOJ mice for the evaluation of the immunogenicity of viral proteins.
在这项研究中，我们用表达丙型肝炎病毒（HCV）包膜蛋白（rMV-E1E2）的麻疹病毒 Edmonston B 株（MV-Edm）感染植入人外周血白细胞（hu-PBL-NOJ）的 NOD/Scid/Jak3null 小鼠，以评估其作为候选疫苗的免疫原性。尽管在为期 2 周的实验期间可以从模拟感染小鼠的脾脏中分离出人白细胞，但感染 MV（10(3)-10(5)pfu）或 rMV-E1E2（10(4)pfu）的小鼠中植入的人白细胞比例降低。通过在脾细胞和 B95a 细胞的共培养物中观察到细胞病变效应（CPE），并通过 RT-PCR 验证，确认了脾细胞的病毒感染。最后，与感染低剂量病毒（MV，10(0)-10(1)pfu 和 rMV-E1E2，10(1)-10(2)pfu）的小鼠的血清中更频繁地观察到针对 MV 的人抗体，而不是针对 E2 的特异性抗体。这些结果表明 hu-PBL-NOJ 小鼠有可能用于评估病毒蛋白的免疫原性。
