Noguchi P D, Johnson J B, O'Donnell R, Petricciani J C
Science. 1978 Mar 3;199(4332):980-3. doi: 10.1126/science.203036.
We used chick embryonic skin (CES) in organ culture to assess the neoplastic potential of a variety of cultured human and nonhuman cell lines. Cells derived from cancer tissues grew in CES and formed tumors in nude mice while cells derived from normal tissues grew in neither system. The CES proved to be more sensitive than the nude mouse when used to assay SV40 transformed human cells; each of four such lines grew in CES while only one of the four lines grew and formed tumors in nude mice. In addition, the patterns of invasion by inoculated cells can be easily studied in the CES. These results suggest that CES in organ culture offers an inexpensive, rapid, and reliable alternative to the nude mouse as a tumorigenicity test.
我们使用鸡胚皮肤（CES）进行器官培养，以评估多种培养的人类和非人类细胞系的肿瘤形成潜力。来自癌组织的细胞在CES中生长，并在裸鼠中形成肿瘤，而来自正常组织的细胞在这两种体系中均不生长。当用于检测SV40转化的人类细胞时，CES被证明比裸鼠更敏感；四个这样的细胞系中的每一个都在CES中生长，而四个细胞系中只有一个在裸鼠中生长并形成肿瘤。此外，接种细胞的侵袭模式在CES中可以很容易地进行研究。这些结果表明，器官培养中的CES作为一种致瘤性测试，为裸鼠提供了一种廉价、快速且可靠的替代方法。
