Fazely F, Moses D C, Ledinko N
In Vitro Cell Dev Biol. 1985 Jul;21(7):409-14. doi: 10.1007/BF02623472.
Invasion of chick chorioallantoic membrane (CAM) organ cultures by rat 3Y1 cells transformed by the highly oncogenic human adenovirus type 12 (3Y1/12-10 cells) was inhibited by several retinoids tested. The anti-invasive activity of the retinoids was dependent on retinoid concentration and continuous (4 d) exposure of the CAM. The 50% retinoid dose (dose effective in achieving a response in half of the organ cultures) that inhibited invasion was 0.85 micrograms/ml of retinol palmitate, 0.39 micrograms/ml of retinoic acid, or 0.16 micrograms/ml of retinol acetate. This dose was of the same order of magnitude as that which induced CAM differentiation, and was three- to fourfold less than the dose that caused cytotoxic damage of CAM. In addition, the retinoids inhibited 3Y1/12-10 cell growth by approximately 40% at levels over 10-fold higher than those needed for anti-invasion activity. The findings suggest that the anti-invasive activity of retinoids was at least partly due to direct induction of cell differentiation of the CAM host tissue.
经高致癌性人12型腺病毒转化的大鼠3Y1细胞（3Y1/12 - 10细胞）对鸡胚绒毛尿囊膜（CAM）器官培养物的侵袭受到了所测试的几种类维生素A的抑制。类维生素A的抗侵袭活性取决于类维生素A的浓度以及对CAM的持续（4天）暴露。抑制侵袭的50%类维生素A剂量（在一半的器官培养物中产生反应的有效剂量）为：棕榈酸视黄酯0.85微克/毫升、视黄酸0.39微克/毫升或醋酸视黄酯0.16微克/毫升。该剂量与诱导CAM分化的剂量处于同一数量级，并且比导致CAM细胞毒性损伤的剂量低三到四倍。此外，类维生素A在比抗侵袭活性所需水平高10倍以上时，对3Y1/12 - 10细胞生长的抑制作用约为40%。这些发现表明，类维生素A的抗侵袭活性至少部分归因于对CAM宿主组织细胞分化的直接诱导。
