Stiles C D, Desmond W, Sato G, Saier M H
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4971-5. doi: 10.1073/pnas.72.12.4971.
Four individual lines and one subline of human cells, permanently established in tissue culture after infection with simian virus 40, failed to form tumors when inoculated into athymic nude mice. Under identical conditions, three established human cell lines of neoplastic origin and a spontaneously established human lymphocyte line formed tumors. Nude mice that failed to grow tumors from inocula of simian virus 40-transformed human cells, grew tumors from subsequent injections of authentic human cancer cells. Further efforts to demonstrate an immunologic basis for the growth suppression of human simian virus 40 transformants were also negative. The data suggest that the changes in morphology and in vitro growth behavior induced by the viral information are not sufficient for, or are only coincidentally related to, the neoplastic state.
用猿猴病毒40感染后在组织培养中永久建立的四个人类细胞系和一个亚系，接种到无胸腺裸鼠体内时未能形成肿瘤。在相同条件下，三个已建立的肿瘤源性人类细胞系和一个自发建立的人类淋巴细胞系形成了肿瘤。未能从猿猴病毒40转化的人类细胞接种物中生长肿瘤的裸鼠，在随后注射真正的人类癌细胞后长出了肿瘤。进一步证明人类猿猴病毒40转化体生长受抑制的免疫学基础的努力也为阴性。数据表明，病毒信息诱导的形态和体外生长行为变化对于肿瘤状态而言是不够的，或者只是偶然相关。
