通过电子顺磁共振波谱检测一氧化氮。
Detection of nitric oxide by electron paramagnetic resonance spectroscopy.
机构信息
Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
出版信息
Free Radic Biol Med. 2010 Jul 15;49(2):122-9. doi: 10.1016/j.freeradbiomed.2010.03.009. Epub 2010 Mar 18.
Abstract
Electron paramagnetic resonance (EPR) spectroscopy has been used in a number of ways to study nitric oxide chemistry and biology. As an intrinsically stable and relatively unreactive diatomic free radical, the challenges of detecting this species by EPR are somewhat different from those of transient radical species. This review gives a basic introduction to EPR spectroscopy and discusses its uses to assess and quantify nitric oxide formation in biological systems.
摘要
电子顺磁共振（EPR）光谱学在许多方面被用于研究一氧化氮的化学和生物学。作为一种本质上稳定且相对不易反应的双原子自由基，通过 EPR 检测这种物质的挑战与瞬态自由基物种有所不同。本文对 EPR 光谱学进行了基本介绍，并讨论了其在评估和量化生物体系中一氧化氮形成中的应用。
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