Sub-department of Environmental Technology, Wageningen University, Biotechnion-Bomenweg 2, PO Box 8129, 6700 EV Wageningen, The Netherlands.
Bioresour Technol. 2010 Jul;101(14):5201-5. doi: 10.1016/j.biortech.2010.02.047. Epub 2010 Mar 20.
The effect of a pulse addition of vitamin B(12) as cobalt source to restore the performance of cobalt depleted methanol-fed bioreactors was investigated. One upflow anaerobic sludge bed (UASB) reactor was supplied with a pulse of vitamin B(12), and its operation was compared to that of another cobalt depleted UASB reactor to which a pulse of CoCl(2) was given. The addition of cobalt in the form of CoCl(2) supplies enough cobalt to restore methanogenesis and maintain full methanol degradation coupled to methane production during more than 35 days after the CoCl(2) pulse. Similar to CoCl(2), pulse addition of vitamin B(12) supplies enough cobalt to maintain full methanol degradation during more than 35 days after the pulse. However, the specific methanogenic activities (SMAs) of the sludge in the vitamin B(12) supplied reactor were around 3 times higher than the SMA of the sludge from the CoCl(2) supplied reactor at the same sampling times. An appropriate dosing strategy (repeated pulse dosing) combined with the choice of vitamin B(12) as the cobalt species is suggested as a promising dosing strategy for methanol-fed anaerobic bioreactors limited by the micronutrient cobalt.
研究了脉冲添加维生素 B(12)作为钴源对恢复钴耗尽的甲醇进料生物反应器性能的影响。一个上流式厌氧污泥床（UASB）反应器被供应了维生素 B(12)脉冲，其运行情况与另一个钴耗尽的 UASB 反应器进行了比较，后者供应了钴氯化物（CoCl(2)）脉冲。以 CoCl(2)的形式添加钴可提供足够的钴，以在 CoCl(2)脉冲后超过 35 天的时间内恢复产甲烷作用并保持完全甲醇降解与甲烷生产相偶联。类似于 CoCl(2)，维生素 B(12)脉冲添加可在脉冲后超过 35 天的时间内提供足够的钴来维持完全甲醇降解。然而，在相同的采样时间，供应维生素 B(12)的反应器中污泥的特定产甲烷活性（SMAs）比供应 CoCl(2)的反应器中污泥的 SMA 高约 3 倍。建议采用适当的投加策略（重复脉冲投加）与选择维生素 B(12)作为钴物质相结合，作为受微量元素钴限制的甲醇进料厌氧生物反应器的一种有前途的投加策略。
