Zollino M, Genuardi M, Tanci P, Mango G, Rumi C, Mancini R, Neri G
Istituti di Genetica Umana, Facoltà di Medicina A. Gemelli, Università Cattolica, Roma, Italy.
Leuk Res. 1991;15(4):269-73. doi: 10.1016/0145-2126(91)90130-l.
We report on a 69-year-old man who developed Ph-positive CML 6 years after the onset of B-cell CLL. When CML was diagnosed, both malignant cell populations were detected in bone marrow and peripheral blood. Peripheral leukocytes were fractionated by Ficoll-Hypaque density gradient, and cytogenetic and molecular studies were performed on mononuclear cell and granulocyte-enriched populations. Mononuclear cells were stimulated with either PHA or PWM. In PHA-treated cultures 76% of the metaphases were Ph-negative, while after PWM stimulation 87% were Ph-positive. A bcr rearrangement was observed in DNA from the granulocyte-enriched fraction, but not in mononuclear cells. On the contrary the IgH locus resulted in monoclonally rearranged DNA, only in peripheral blood mononuclear cells. These results indicate that the two neoplastic populations originated independently.
我们报告了一名69岁男性，他在B细胞慢性淋巴细胞白血病（CLL）发病6年后发生了Ph阳性慢性髓性白血病（CML）。当诊断出CML时，在骨髓和外周血中均检测到两种恶性细胞群。通过Ficoll-Hypaque密度梯度对外周血白细胞进行分离，并对单核细胞和粒细胞富集群体进行细胞遗传学和分子研究。单核细胞用PHA或PWM刺激。在PHA处理的培养物中，76%的中期细胞为Ph阴性，而在PWM刺激后，87%为Ph阳性。在粒细胞富集部分的DNA中观察到bcr重排，但在单核细胞中未观察到。相反，仅在外周血单核细胞中，IgH基因座导致DNA单克隆重排。这些结果表明这两个肿瘤群体是独立起源的。
