Maher V E, Gill L, Townes P L, Wallace J E, Savas L, Woda B A, Ansell J E
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655.
Cancer. 1993 Mar 15;71(6):1993-7. doi: 10.1002/1097-0142(19930315)71:6<1993::aid-cncr2820710612>3.0.co;2-6.
The authors studied a patient with the simultaneous occurrence of chronic lymphocytic leukemia (CLL) and chronic myelogenous leukemia (CML). The coexistence of these two hematologic malignancies leads to questions about their cell of origin. Through analysis of this patient's DNA, the authors studied the derivation of the two malignancies. They separated the blood into a myeloid-rich fraction and a fraction containing the malignant lymphocytes. JH and bcr probes were used to study these loci in the myeloid and lymphoid fractions and in unfractionated white blood cells. The authors found that the unfractionated leukocytes contained the bcr and JH rearrangements. Conversely, the lymphoid fraction contained only the JH rearrangement, and the myeloid fraction contained only the bcr rearrangement, suggesting that these malignancies arose from separate stem cells. This is the first reported patient with simultaneously occurring CML and CLL definitively shown to arise from distinct progenitors, and this report raises questions about the origin of these two cell lines.
作者研究了一名同时患有慢性淋巴细胞白血病（CLL）和慢性粒细胞白血病（CML）的患者。这两种血液系统恶性肿瘤的共存引发了关于它们起源细胞的问题。通过对该患者DNA的分析，作者研究了这两种恶性肿瘤的起源。他们将血液分离为富含髓细胞的部分和含有恶性淋巴细胞的部分。使用JH和bcr探针研究这些位点在髓细胞和淋巴细胞部分以及未分离的白细胞中的情况。作者发现未分离的白细胞含有bcr和JH重排。相反，淋巴细胞部分仅含有JH重排，而髓细胞部分仅含有bcr重排，这表明这些恶性肿瘤起源于不同的干细胞。这是首例报告的同时发生CML和CLL且明确显示起源于不同祖细胞的患者，该报告引发了关于这两种细胞系起源的问题。
