Advanced Photonics Laboratory (MVD), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
J Biophotonics. 2010 Jul;3(7):432-6. doi: 10.1002/jbio.201000018.
The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell parameters including absolute volume, shape and integral intracellular refractive index. In combination with the numerous different specific fluorescent cellular probes, this multimodality microscopy can address important issues in cell biology. This is demonstrated by the study of intracellular calcium homeostasis associated with the change in cell volume, which play a critical role in the excitotoxicity-induced neuronal death.
作者开发了一种活细胞多模式显微镜，将荧光显微镜与数字全息显微镜相结合；该显微镜采用解耦程序，可以分别测量定量相位中重要的细胞参数，包括绝对体积、形状和整体细胞内折射率。结合众多不同的特定荧光细胞探针，这种多模式显微镜可以解决细胞生物学中的重要问题。这一点通过研究与细胞体积变化相关的细胞内钙稳态得到了证明，细胞内钙稳态在兴奋性毒性诱导的神经元死亡中起着关键作用。
